Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Inducible long-term gene expression in brain with adeno-associated virus gene transfer
Autore:
Haberman, RP; McCown, TJ; Samulski, RJ;
Indirizzi:
Univ N Carolina, UNC Gene Therapy Ctr, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599 y Ctr, Chapel Hill, NC 27599 USA Univ N Carolina, Ctr Neurosci, Chapel Hill, NC USA Univ N Carolina ChapelHill NC USA na, Ctr Neurosci, Chapel Hill, NC USA Univ N Carolina, Dept Psychiat, Chapel Hill, NC USA Univ N Carolina Chapel Hill NC USA a, Dept Psychiat, Chapel Hill, NC USA Univ N Carolina, Dept Pharmacol, Chapel Hill, NC USA Univ N Carolina Chapel Hill NC USA , Dept Pharmacol, Chapel Hill, NC USA Univ N Carolina, Curriculum Neurobiol, Chapel Hill, NC USA Univ N Carolina Chapel Hill NC USA iculum Neurobiol, Chapel Hill, NC USA
Titolo Testata:
GENE THERAPY
fascicolo: 12, volume: 5, anno: 1998,
pagine: 1604 - 1611
SICI:
0969-7128(199812)5:12<1604:ILGEIB>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
TETRACYCLINE-RESPONSIVE PROMOTER; TRANSGENIC MICE; MAMMALIAN-CELLS; AAV VECTOR; SYSTEM; INTEGRATION; EFFICIENT; NEURONS;
Keywords:
gene transfer; gene regulation; adeno-associated virus; neurons;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Samulski, RJ Univ N Carolina, UNC Gene Therapy Ctr, CB 7352,7119 Thurston Bowles, Chapel Univ N Carolina CB 7352,7119 Thurston Bowles Chapel Hill NC USA 27599
Citazione:
R.P. Haberman et al., "Inducible long-term gene expression in brain with adeno-associated virus gene transfer", GENE THER, 5(12), 1998, pp. 1604-1611

Abstract

Recombinant adeno-associated virus (rAAV) vectors hold promise for treating a number of neurological disorders due to the ability to deliver long-term gene expression without toxicity or immune response. Critical to these endeavors will be controlled expression of the therapeutic gene in target We have constructed and tested a dual cassette rAAV vector carrying a reportergene under the control of the tetracycline-responsive system and the tetracycline transactivator. Transduction in vitro resulted in stable expressionfrom the vector that can be suppressed 20-fold by tetracycline treatment. In vivo experiments, carried out to 6 weeks, demonstrated that vector-transduced expression is sustained until doxycycline administration upon which reporter gene expression is reduced. Moreover, the suppression of vector-driven expression can be reversed by removal of the drug. These studies demonstrate long-term regulated gene expression from rAAV vectors. This system will provide a valuable approach for controlling vector gene expression both in vitro and in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 15:47:17