Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Neutrophil chemokines in bronchoalveolar lavage fluid and leukocyte-conditioned medium from nonsmokers and smokers
Autore:
Morrison, D; Strieter, R; Donnelly, SC; Burdick, MD; Kunkel, SL; MacNee, W;
Indirizzi:
Univtlandburgh, Dept Med, Resp Med Unit, Edinburgh EH8 9YL, Midlothian, Sco Univ Edinburgh Edinburgh Midlothian Scotland EH8 9YL 9YL, Midlothian, Sco Univ Michigan, Dept Pathol, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 Dept Pathol, Ann Arbor, MI 48109 USA Univ Michigan, Dept Pulm & Crit Care, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 & Crit Care, Ann Arbor, MI 48109 USA
Titolo Testata:
EUROPEAN RESPIRATORY JOURNAL
fascicolo: 5, volume: 12, anno: 1998,
pagine: 1067 - 1072
SICI:
0903-1936(199811)12:5<1067:NCIBLF>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESPIRATORY-DISTRESS-SYNDROME; IDIOPATHIC PULMONARY FIBROSIS; INTERLEUKIN-8 GENE-EXPRESSION; HUMAN-ENDOTHELIAL-CELLS; CIGARETTE-SMOKING; PNEUMOCYSTIS-CARINII; CHEMOTACTIC FACTOR; LUNG INFLAMMATION; CYTOKINES; MACROPHAGES;
Keywords:
Bronchoalveolar lavage fluid; chemotactic factors; lipopolysaccharides; polymorphonuclear leukocytes; smoking;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: MacNee, W Royalandfirm, Dept Med, Resp Med Unit, Edinburgh EH3 9YW, Midlothian, Scotl Royal Infirm Edinburgh Midlothian Scotland EH3 9YW thian, Scotl
Citazione:
D. Morrison et al., "Neutrophil chemokines in bronchoalveolar lavage fluid and leukocyte-conditioned medium from nonsmokers and smokers", EUR RESP J, 12(5), 1998, pp. 1067-1072

Abstract

Polymorphonuclear neutrophils (PMN) have been implicated in the pathogenesis of emphysema, The chemokines interleukin-8 (IL-8), growth-related oncogene (GRO-alpha) and extractable nuclear antigen (ENA)-78 may be involved in the increased numbers of PMN in smokers' airspaces. The levels of these cytokines in bronchoalveolar lavage fluid (BALF) and bronchoalveolar lavage leukocyte conditioned medium (LCM), along with BALF PMN numbers in 12 smokers who abstained for 12 h (chronic smoking) or continued to smoke until 1 h before study (acute smoking) and seven nonsmokers were compared. Neutrophils in BALF increased in acute (1.96+/-0.53%, 0.99+/-0.32x10(6) cells:) compared with chronic smokers (0.59+/-0.25%, 0.61+/-0.24x10(6) cells,p<0.05 nonsmokers) and nonsmokers (0.79+/-0.29 %, 0.05+/-0.01x10(6) cells,p<0.05),There were no differences in IL-8 or GRO-alpha in BALF between smokers and nonsmokers. ENA-78 levels were lower in smokers (p=0.006), There was no difference in IL-8, GRO-alpha or ENA-78 in LCM from unstimulated cells in smokers versus nonsmokers. After stimulation with lipopolysaccharide (LPS) 10 ng.mL(-1), IL-8 release in acute smokers (p=0.04) and GRO-alpha release in smokers (p=0.009) were significantly higher than in nonsmokers. Following stimulation with LPS 100 ng.mL(-1), GRO-alpha release was higher in smokers (p=0.03) and increased further in acute smokers (p=0.02 versus nonsmokers, p=0.04 versus chronic smokers) and ENA-78 release increased in smokers (p=0.02 versus nonsmokers),In conclusion, influx of polymorphonuclear neutrophils into smokers' airspaces is an acute phenomenon and neutrophil chemokine release from mixed bronchoalveolar lavage leukocytes is influenced by cigarette smoking and endotoxins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 10:19:56