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Titolo:
Human prostaglandin transporter gene (hPGT) is regulated by fluid mechanical stimuli in cultured endothelial cells and expressed in vascular endothelium in vivo
Autore:
Topper, JN; Cai, JX; Stavrakis, G; Anderson, KR; Woolf, EA; Sampson, BA; Schoen, FJ; Falb, D; Gimbrone, MA;
Indirizzi:
Stanfordtanford,ch Med, Dept Med, Div Cardiovasc,Falk Cardiovasc Res Ctr, S Stanford Univ Stanford CA USA 94305 Cardiovasc,Falk Cardiovasc Res Ctr, S Harvard,Univ, Sch Med, Div Vasc Res, Brigham & Womens Hosp,Dept Med, Boston Harvard Univ Boston MA USA c Res, Brigham & Womens Hosp,Dept Med, Boston Harvard Univ, Sch Med, Dept Pathol, Brigham & Womens Hosp,Dept Med, Boston, Harvard Univ Boston MA USA thol, Brigham & Womens Hosp,Dept Med, Boston, Harvardon,iv, Sch Med, Div Cardiovasc, Brigham & Womens Hosp,Dept Med, Bost Harvard Univ Boston MA USA diovasc, Brigham & Womens Hosp,Dept Med, Bost Millennium Pharmaceut Inc, Cambridge, MA USA Millennium Pharmaceut Inc Cambridge MA USA maceut Inc, Cambridge, MA USA
Titolo Testata:
CIRCULATION
fascicolo: 22, volume: 98, anno: 1998,
pagine: 2396 - 2403
SICI:
0009-7322(199812)98:22<2396:HPTG(I>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEAR MATRIX; IDENTIFICATION; CLONING; FLOW;
Keywords:
prostaglandins; cardiovascular system; stress; endothelium; gene expression; carrier proteins;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Topper, JN Stanford00niv, Sch Med, Dept Med, Div Cardiovasc,Falk Cardiovasc Res Ctr, 3 Stanford Univ 300 Pasteur Dr Stanford CA USA 94305 c Res Ctr, 3
Citazione:
J.N. Topper et al., "Human prostaglandin transporter gene (hPGT) is regulated by fluid mechanical stimuli in cultured endothelial cells and expressed in vascular endothelium in vivo", CIRCULATION, 98(22), 1998, pp. 2396-2403

Abstract

Background-biomechanical forces generated by blood flow within the cardiovascular system have been proposed as important modulators of regional endothelial phenotype and function. This process is thought to involve the regulation of vascular gene expression by physiological fluid mechanical stimulisuch as fluid sheer stresses. Methods and Results-We demonstrate sustained upregulation of a recently identified gene encoding a human prostaglandin transporter (hPGT) in culturedhuman vascular endothelium exposed to a physiological fluid mechanical stimulus in vitro. This biomechanical induction is selective in that steady laminar shear stress is sufficient to upregulate the hPGT gene at the level of transcriptional activation, whereas a comparable level of turbulent sheerstress (a nonphysiological stimulus) is not. Various biochemical stimuli, such as bacterial endotoxin and the inflammatory cytokines recombinant human interleukin 1 beta cytokines (rhIL-1 beta) and tumor necrosis factor-alpha (TNF-alpha), did not significantly induce hPGT. Using a specific antiserum to hPGT, we demonstrate endothelial expression within the arterial vasculature and the microcirculation of highly vascularized tissues such as the heart. Conclusions-Our results identify hPGT as an inducible gene in vascular endothelium and suggest that biomechanical stimuli generated by blood flow in vivo may be important determinants of hPGT expression. Furthermore, this demonstration of regulated endothelial expression of hPGT implicates this molecule in the regional metabolism of prostanoids within the cardiovascular system.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 09:42:04