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Titolo:
The augmentation of melanocytic nevi in guinea pigs by solar-simulated light: An animal model for human melanocytic nevi
Autore:
Menzies, S; Khalil, M; Crotty, K; Bonin, A;
Indirizzi:
UnivWSydney, Royal Prince Alfred Hosp, Sydney Melanoma Unit, Camperdown, NS Univ Sydney Camperdown NSW Australia 2050 y Melanoma Unit, Camperdown, NS Univ Sydney, Dept Pathol, Sydney, NSW 2006, Australia Univ Sydney Sydney NSW Australia 2006 Pathol, Sydney, NSW 2006, Australia Natl Occupat Hlth & Safety Commiss, Camperdown, NSW 2050, Australia Natl Occupat Hlth & Safety Commiss Camperdown NSW Australia 2050 ustralia
Titolo Testata:
CANCER RESEARCH
fascicolo: 23, volume: 58, anno: 1998,
pagine: 5361 - 5366
SICI:
0008-5472(199812)58:23<5361:TAOMNI>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
CUTANEOUS MALIGNANT-MELANOMA; ULTRAVIOLET-RADIATION; MONODELPHIS-DOMESTICA; TRANSGENIC MICE; DNA PHOTOLYASE; HAIRLESS MICE; SKIN MELANOMA; TUMORS; INDUCTION; CHILDREN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Menzies, S UnivWSydney, Royal Prince Alfred Hosp, Sydney Melanoma Unit, Camperdown, NS Univ Sydney Camperdown NSW Australia 2050 Unit, Camperdown, NS
Citazione:
S. Menzies et al., "The augmentation of melanocytic nevi in guinea pigs by solar-simulated light: An animal model for human melanocytic nevi", CANCER RES, 58(23), 1998, pp. 5361-5366

Abstract

Strong epidemiological evidence confirms the role of sunlight in human melanoma induction. Furthermore, the frequency of melanocytic nevi is a good indicator of future development of melanoma and a short-term marker of adverse reactions to melanoma-inducing sun exposure in humans. Thus, the aim of this study was to develop and define an animal model for sunlight-induced nevi that can be used as a surrogate model for sunlight-induced melanoma. Five treatment groups of 30-40 Hartley albino guinea pigs/group were treated with topical 7,12-dimethylbenzanthracene at a dose range of 6-240 mg on thedorsum of the skin. At week 20, half of the animals in each group were given a 12-month regimen of minimal erythemal solar-simulated Light, 3 times/week, increased weekly to maintain erythema, These regimes induced epidermally derived pigmented melanocytic nevi clinically and histologically similarto human nevi (junctional, compound, and dermal), S100 and HMB45 staining was also consistent with the patterns seen in human nevi. In contrast to the high-dose 7,12-dimethylbenzanthracene-treated animals (60 and 240 mg), where solar-simulated light had no effect on nevi multiplicity, those groups treated with low doses (24, 12, and 6 mg) had a significant increase in nevi multiplicity after 12 months of solar-simulated light treatment (24 mg, 0.5 nevi/animal unirradiated versus 1.4 nevi/animal irradiated, P = 0.03; 12mg, 0.2 unirradiated versus 1.2 irradiated, P = 0.02; 6 mg, 0.2 unirradiated versus 1.9 irradiated, P = 0.008). UVB-induced minimal erythemal dose was unaltered after exposure to photoreactivating light, consistent with the observation of others that placental mammals lack the DNA photolyase responsible for strong photoreactivation seen in nonplacental mammals and lower metazoans, Thus, our guinea pig model has some of the essential elements required to be a robust animal model for human nevi and a surrogate model for melanoma, These nevi are augmented by solar-simulated light, are histologically similar, occupy the same level within the skin, have the same natural history as human nevi, and are produced in an animal lacking strong photoreactivation. These features are not found in any previously described small laboratory animal model.

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Documento generato il 27/11/20 alle ore 06:35:16