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Titolo:
A novel catechol oxidase enzyme electrode for the specific determination of catechol
Autore:
Dinckaya, E; Akyilmaz, E; Akgol, S; Onal, ST; Zihnioglu, F; Telefoncu, A;
Indirizzi:
Ege Univ, Fac Sci, Dept Biochem, TR-35100 Izmir, Turkey Ege Univ Izmir Turkey TR-35100 Sci, Dept Biochem, TR-35100 Izmir, Turkey
Titolo Testata:
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
fascicolo: 11, volume: 62, anno: 1998,
pagine: 2098 - 2100
SICI:
0916-8451(199811)62:11<2098:ANCOEE>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESTROGENS;
Keywords:
enzyme electrode; biosensor; catechol; eggplant; (Solanum melangena L.);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: Dinckaya, E Ege Univ, Fac Sci, Dept Biochem, TR-35100 Izmir, Turkey Ege Univ Izmir Turkey TR-35100 ochem, TR-35100 Izmir, Turkey
Citazione:
E. Dinckaya et al., "A novel catechol oxidase enzyme electrode for the specific determination of catechol", BIOS BIOT B, 62(11), 1998, pp. 2098-2100

Abstract

An enzyme electrode for the specific determination of catechol was developed by using catechol oxidase (EC 1.10.3.1) from eggplant (Solanum melangenaL.) in combination with a dissolved oxygen probe. Optimization studies of the prepared catechol oxidase enzyme electrode established a phosphate buffer 50 mM at pH 7.0 and 35 degrees C to provide the optimum conditions for affirmative electrode response. The enzyme electrode response depended linearly on a catechol concentration range of 5.10(-7)-30.10(-5) M with a response time of 25 sec and substrate specificity of the catechol oxidase electrode of 100%. The biosensor retained its enzyme activity for at least 70 days.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/08/20 alle ore 14:28:52