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Titolo:
Use of C-13 tags with specifically N-15-labeled DNA and RNA
Autore:
Abad, JL; Shallop, AJ; Gaffney, BL; Jones, RA;
Indirizzi:
Rutgers State Univ, Dept Chem, Piscataway, NJ 08855 USA Rutgers State Univ Piscataway NJ USA 08855 Chem, Piscataway, NJ 08855 USA
Titolo Testata:
BIOPOLYMERS
fascicolo: 1, volume: 48, anno: 1998,
pagine: 57 - 63
SICI:
0006-3525(1998)48:1<57:UOCTWS>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
SELF-SPLICING INTRONS; NITROGEN-15-LABELED OLIGODEOXYNUCLEOTIDES; N-15 NMR; BASE-PAIR; DEOXYNUCLEOSIDES; DEOXYADENOSINE; RECOGNITION; NUCLEOSIDES; CLEAVAGE; BINDING;
Keywords:
N-15-nmr; C-13-nmr; specific labeling; C-13 tags; nucleoside synthesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Jones, RA Rutgers State Univ, Dept Chem, POB 939, Piscataway, NJ 08855 USARutgers State Univ POB 939 Piscataway NJ USA 08855 NJ 08855 USA
Citazione:
J.L. Abad et al., "Use of C-13 tags with specifically N-15-labeled DNA and RNA", BIOPOLYMERS, 48(1), 1998, pp. 57-63

Abstract

We describe methods to introduce C-13 specifically to the C2 or C8 positions of N-15-multilabeled purines, thereby permitting C-13 tagging" of all base nitrogens except the amino group of adenosine. These procedures permit the incorporation of two or more N-15-multilabeled monomers into a given DNAor RNA fragment, with and without appropriate C-13 tags. This approach increases the number of N-15-nmr resonances that can be unambiguously distinguished and thus the amount of information from a single synthesis and a single nmr experiment. (C) 1998 John Wiley & Sons, Inc.

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Documento generato il 03/07/20 alle ore 01:33:23