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Titolo:
CIS-ACTING ELEMENTS ESSENTIAL FOR LIGHT REGULATION OF THE NUCLEAR GENE ENCODING THE A-SUBUNIT OF CHLOROPLAST GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE IN ARABIDOPSIS-THALIANA
Autore:
PARK SC; KWON HB; SHIH MC;
Indirizzi:
UNIV IOWA,DEPT BIOL SCI IOWA CITY IA 52242 UNIV IOWA,DEPT BIOL SCI IOWA CITY IA 52242
Titolo Testata:
Plant physiology
fascicolo: 4, volume: 112, anno: 1996,
pagine: 1563 - 1571
SICI:
0032-0889(1996)112:4<1563:CEEFLR>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
RBCS-3A GENE; PROTEIN INTERACTIONS; EXPRESSION; BINDING; PHYTOCHROME; SEQUENCES; PROMOTER; CARBOXYLASE; UPSTREAM; PLANTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
S.C. Park et al., "CIS-ACTING ELEMENTS ESSENTIAL FOR LIGHT REGULATION OF THE NUCLEAR GENE ENCODING THE A-SUBUNIT OF CHLOROPLAST GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE IN ARABIDOPSIS-THALIANA", Plant physiology, 112(4), 1996, pp. 1563-1571

Abstract

We report the characterization of cis-acting elements involved in light regulation of the nuclear gene (GapA) that encodes the A subunit ofglyceraldehyde 3-phosphate dehydrogenase in Arabidopsis thaliana. Ourprevious deletion analyses indicate that the -277 to -195 upstream region of GapA is essential for light induction of the p-glucuronidase reporter gene in transgenic tobacco (Nicotiana tabacum) plants. This region contains three direct repeats with the consensus sequence 5'-CAAATGAA(A/G)A-3' (Gap boxes). Our results show that 2-bp substitutions ofthe last four nucleotides (AA or CA) of the Gap boxes by CC abolish light induction of the beta-glucuronidase reporter gene in vivo and affect binding of the Gap box binding factor in vitro. We have also identified an additional cis-acting element, AE (Activation Element) box, that is involved in regulation of GapA. A combination of a Gap box trimer and an AE box dimer can confer light responsiveness on the cauliflower mosaic virus 35S promoter containing the -92 to +6 upstream sequence, whereas oligomers of Gap boxes or AE boxes alone cannot confer light responsiveness on the same promoter. These results suggest that Gapboxes and AE boxes function together as the light-responsive element of GapA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 20:25:05