Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
HIGH-LEVEL RESISTANCE TO (-) ENANTIOMERIC 2'-DEOXY-3'-THIACYTIDINE IN-VITRO IS DUE TO ONE AMINO-ACID SUBSTITUTION IN THE CATALYTIC SITE OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REVERSE-TRANSCRIPTASE
Autore:
BOUCHER CAB; CAMMACK N; SCHIPPER P; SCHUURMAN R; ROUSE P; WAINBERG MA; CAMERON JM;
Indirizzi:
UNIV AMSTERDAM,ACAD MED CTR,ANTIVIRAL THERAPY LAB,MEIBERGDREEF 15 1105 AZ AMSTERDAM NETHERLANDS UNIV AMSTERDAM,ACAD MED CTR,ANTIVIRAL THERAPY LAB,MEIBERGDREEF 15 1105 AZ AMSTERDAM NETHERLANDS GLAXO GRP RES LTD GREENFORD UB6 0HE MIDDX ENGLAND SIR MORTIMER B DAVIS JEWISH HOSP,LADY DAVIS INST MONTREAL H3T 1E2 QUEBEC CANADA MCGILL UNIV,CTR AIDS MONTREAL H3T 1E2 PQ CANADA
Titolo Testata:
Antimicrobial agents and chemotherapy
fascicolo: 10, volume: 37, anno: 1993,
pagine: 2231 - 2234
SICI:
0066-4804(1993)37:10<2231:HRT(E2>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
REPLICATION INVITRO; ZIDOVUDINE; ASSAY; MUTATION; SENSITIVITY; INHIBITOR; INFECTION; TOXICITY; BCH-189; MUTANTS;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
C.A.B. Boucher et al., "HIGH-LEVEL RESISTANCE TO (-) ENANTIOMERIC 2'-DEOXY-3'-THIACYTIDINE IN-VITRO IS DUE TO ONE AMINO-ACID SUBSTITUTION IN THE CATALYTIC SITE OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REVERSE-TRANSCRIPTASE", Antimicrobial agents and chemotherapy, 37(10), 1993, pp. 2231-2234

Abstract

Passage of human immunodeficiency virus type 1 in the presence of increasing 2'-deoxy-3'-thiacytidine (3TC) concentrations results in high-level (> 100-fold) 3TC-resistant viruses. All 3TC-resistant viruses possess a substitution at the second codon (from a methionine into an isoleucine) at position 184 within the highly conserved motif (YMDD) of human immunodeficiency virus type 1 reverse transcriptase. 3TC-resistant viruses were cross-resistant to the (-) enantiomer of the fluorinated derivative of BCH-189 but remained susceptible to 2',3'-dideoxyinosine and 2',3'-dideoxycytidine. The susceptibilities of the 3TC-resistant viruses to the (+) enantiomers of BCH-189 and the fluorinated derivative of BCH-189 demonstrate an enantiomeric specificity for viruses selected under these conditions. Introduction of an isoleucine substitution at codon 184 into a background of two known 3'-azido-3'-deoxythymidine resistance mutations (amino acids 41 and 215) restored the susceptibility of this virus to 3'-azido-3'-deoxythymidine.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 15:39:13