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Titolo:
VALIDATION OF REFLECTANCE INFRARED-SPECTROSCOPY AS A QUANTITATIVE METHOD TO MEASURE PERCUTANEOUS-ABSORPTION IN-VIVO
Autore:
HIGO N; NAIK A; BOMMANNAN DB; POTTS RO; GUY RH;
Indirizzi:
UNIV CALIF SAN FRANCISCO,SCH PHARM SAN FRANCISCO CA 94143 HISAMITSU PHARMACEUT CO INC TSUKUBA IBARAKI 305 JAPAN CYGNUS THERAPEUT SYST REDWOOD CITY CA 94063 UNIV CALIF SAN FRANCISCO,DEPT PHARM SAN FRANCISCO CA 94143 UNIV CALIF SAN FRANCISCO,DEPT PHARMACEUT CHEM SAN FRANCISCO CA 94143
Titolo Testata:
Pharmaceutical research
fascicolo: 10, volume: 10, anno: 1993,
pagine: 1500 - 1506
SICI:
0724-8741(1993)10:10<1500:VORIAA>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
STRATUM-CORNEUM; OLEIC-ACID;
Keywords:
SKIN PENETRATION; PENETRATION ENHANCEMENT; OLEIC ACID; 4-CYANOPHENOL; INFRARED SPECTROSCOPY; TRANSDERMAL DELIVERY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
N. Higo et al., "VALIDATION OF REFLECTANCE INFRARED-SPECTROSCOPY AS A QUANTITATIVE METHOD TO MEASURE PERCUTANEOUS-ABSORPTION IN-VIVO", Pharmaceutical research, 10(10), 1993, pp. 1500-1506

Abstract

Attenuated total-reflectance infrared (ATR-IR) spectroscopy has been used to follow the penetration of a model compound (4-cyanophenol; CP)across human stratum corneum (SC) in vivo, in man. CP was administered for periods of 1, 2, or 3 hr, either (a) as a 10% (w/v) solution in propylene glycol or (b) in an identical vehicle which also contained 5% (v/v) oleic (cis-9-octadecenoic) acid. At the end of the treatment periods, SC at the application site was progressively removed by adhesive tape-stripping. Prior to the removal of the first tape-strip, and after each subsequent tape-strip, an ATR-IR spectrum of the treated site was recorded. The presence of CP, as a function of position in the SC, was monitored spectroscopically via the intense C=N stretching absorbance at 2230 cm-1. The absolute amount of CP, as a function of SC depth, was determined by ''spiking'' the applied solutions with C-14-labeled compound and subsequent liquid scintillation counting of the removed tape-strips. The presence of oleic acid in the applied formulationsignificantly increased the rate and extent of CP delivery as evaluated by either spectroscopy or radiochemical analysis. Furthermore, the ATR-IR and direct C-14 analysis of CP as a function of SC position were highly correlated. These data strongly support, therefore, the validation of ATR-IR as a quantitative tool to assess percutaneous penetration in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/08/20 alle ore 14:16:25