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Titolo:
ACTIN POLYMERIZATION IN BOAR SPERMATOZOA - FERTILIZATION IS REDUCED WITH USE OF CYTOCHALASIN-D
Autore:
CASTELLANICERESA L; MATTIOLI M; RADAELLI G; BARBONI B; BRIVIO MF;
Indirizzi:
UNIV MILAN,DEPT BIOL,ZOOL & CYTOL SECT,VIA CELORIA 26 I-20133 MILAN ITALY UNIV BOLOGNA,DEPT VET PHYSIOL I-40126 BOLOGNA ITALY
Titolo Testata:
Molecular reproduction and development
fascicolo: 2, volume: 36, anno: 1993,
pagine: 203 - 211
SICI:
1040-452X(1993)36:2<203:APIBS->2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHLORTETRACYCLINE FLUORESCENCE ASSAY; GUINEA-PIG SPERMATOZOA; MAMMALIAN SPERM HEADS; INVITRO CAPACITATION; ACROSOME REACTION; HAMSTER SPERM; PRONUCLEUS FORMATION; DOMESTIC PIG; F-ACTIN; CELLS;
Keywords:
CAPACITATION; IN-VITRO FERTILIZATION; F-ACTIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
L. Castellaniceresa et al., "ACTIN POLYMERIZATION IN BOAR SPERMATOZOA - FERTILIZATION IS REDUCED WITH USE OF CYTOCHALASIN-D", Molecular reproduction and development, 36(2), 1993, pp. 203-211

Abstract

The aggregational state of actin in boar spermatozoa after capacitation and the acrosome reaction has been examined by several methods. In vitro fertilization (IVF) experiments were conducted in the presence and absence of cytochalasin D (CD) to evaluate the role of actin polymerization in the events of fertilization. The fertilizing capacity was very high in controls, but, when CD (an inhibitor of the polymerization of actin) was added to the capacitation medium, there was a marked decrease in the fertilizing capacity of the boar spermatozoa. There wasa further decrease when CD was present during both capacitation and fertilization processes. In addition to the IVF tests, biochemical and immunoelectron microscopic methods were used to analyze the state of aggregation of actin in boar spermatozoa after capacitation, and the acrosome reaction. By immunoelectron microscopy with a phalloidin probe,there were no gold particles, indicating the presence of F-actin on boar sperm heads capacitated and acrosome-reacted in media containing CD. By sodium dodecyl sulfate-polyacrylamide gel electrophoresis there were differences in NP-40 solubility, reflecting actin polymerization,between CD-treated and untreated sperm. These results suggest that actin polymerizes during capacitation and the acrosome reaction and thatthis polymerization is essential to the fertilization process. (C) 1993 Wiley-Liss, Inc.

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Documento generato il 29/11/20 alle ore 02:44:35