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Titolo:
DIFFERENCES IN LDL RECEPTOR-MEDIATED METABOLISM OF 3 LOW-DENSITY-LIPOPROTEIN SUBFRACTIONS BY HUMAN MONOCYTE-DERIVED MACROPHAGES - IMPACT ONTHE RISK FOR ATHEROSCLEROSIS
Autore:
DEGRAAF J; HENDRIKS JCM; SWINKELS DW; DEMACKER PNM; STALENHOEF AFH;
Indirizzi:
UNIV HOSP NIJMEGEN,DEPT GEN INTERNAL MED,POB 9101 6500 HB NIJMEGEN NETHERLANDS UNIV HOSP NIJMEGEN,DEPT STAT CONSULTAT NIJMEGEN NETHERLANDS UNIV HOSP NIJMEGEN,DEPT MED,DIV GEN INTERNAL MED NIJMEGEN NETHERLANDS
Titolo Testata:
Artery
fascicolo: 4, volume: 20, anno: 1993,
pagine: 201 - 230
SICI:
0098-6127(1993)20:4<201:DILRMO>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
CORONARY-ARTERY DISEASE; FAMILIAL HYPERCHOLESTEROLEMIA; SCAVENGER RECEPTOR; APOLIPOPROTEIN-B; GRADIENT ULTRACENTRIFUGATION; MYOCARDIAL-INFARCTION; CULTURED FIBROBLASTS; BEZAFIBRATE THERAPY; HUMAN-PLASMA; CHOLESTEROL;
Keywords:
LOW DENSITY LIPOPROTEIN SUBFRACTIONS; CELL SPECIFIC ASSOCIATION; CELL SPECIFIC DEGRADATION; CHOLESTERYL ESTERIFICATION; LDL RECEPTOR; HUMAN MONOCYTE-DERIVED MACROPHAGES; ATHEROSCLEROSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
J. Degraaf et al., "DIFFERENCES IN LDL RECEPTOR-MEDIATED METABOLISM OF 3 LOW-DENSITY-LIPOPROTEIN SUBFRACTIONS BY HUMAN MONOCYTE-DERIVED MACROPHAGES - IMPACT ONTHE RISK FOR ATHEROSCLEROSIS", Artery, 20(4), 1993, pp. 201-230

Abstract

The metabolism of three low density lipoprotein (LDL) subfractions byhuman monocyte-derived macrophages (HMDM) through the LDL receptor pathway was studied. The three LDL subfractions, very light LDL1, light LDL2 and heavy LDL3, were isolated from serum pools of normolipidemic subjects by density gradient ultracentrifugation. The LDL subfractionswere shown to differ in molecular size and chemical composition but not in electrophoretic mobility on agarose gel. Cell specific association, cell specific degradation and stimulation of cholesteryl esterification were determined in parallel after incubation of HMDM with increasing amounts of LDL-protein of the three LDL subfractions. The experiments were repeated four times with freshly prepared LDL subfractions. Both the cell specific association and degradation increased more withincreasing LDL-protein concentration for LDL1 than for LDL3 (p<0.001). The results for LDL2 were intermediate between those for LDL1 and LDL3 and differed significantly from both (p<0.05). For the stimulation of cholesteryl ester formation, the curves for LDL1 and LDL2 increasedmore with increasing LDL-protein concentration than that for LDL3 (p < 0.001); the results for LDL1 and LDL2 did not differ significantly from each other. These differences between LDL subfractions in cholesteryl esterification were independent of the cholesterol content of the LDL subfractions. The results show that LDL subfractions have different rates of LDL receptor-mediated catabolism by HMDM. As HMDM play an important role in the formation of atherosclerotic plaques, the differences between the LDL subfractions in catabolism by HMDM, may result indifferences in atherogenicity between LDL subfractions isolated from normolipidemic subjects.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/02/20 alle ore 10:47:08