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Titolo:
ORDERING OF PROBES SURROUNDING THE EWINGS-SARCOMA BREAKPOINT ON CHROMOSOME-22 USING FLUORESCENT IN-SITU HYBRIDIZATION TO INTERPHASE NUCLEI
Autore:
SHIPLEY JM; JONES TA; PATEL K; KIELY FM; DESTAVOLA BL; SHEER D;
Indirizzi:
IMPERIAL CANC RES FUND,HUMAN CYTOGENET LAB,44 LINCOLNS INN FIELDS LONDON WC2A 3PX ENGLAND IMPERIAL CANC RES FUND,HUMAN CYTOGENET LAB,44 LINCOLNS INN FIELDS LONDON WC2A 3PX ENGLAND IMPERIAL CANC RES FUND,MED STAT LAB LONDON WC2A 3PX ENGLAND
Titolo Testata:
Cytogenetics and cell genetics
fascicolo: 3-4, volume: 64, anno: 1993,
pagine: 233 - 239
SICI:
0301-0171(1993)64:3-4<233:OOPSTE>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
LEUKEMIA INHIBITORY FACTOR; HUMAN INTERLEUKIN; DNA-SEQUENCES; CELL-NUCLEI; RESOLUTION; TRANSLOCATION; GENE; DISTANCES; CHROMATIN; COSMIDS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
J.M. Shipley et al., "ORDERING OF PROBES SURROUNDING THE EWINGS-SARCOMA BREAKPOINT ON CHROMOSOME-22 USING FLUORESCENT IN-SITU HYBRIDIZATION TO INTERPHASE NUCLEI", Cytogenetics and cell genetics, 64(3-4), 1993, pp. 233-239

Abstract

Eight probes were localized by fluorescent in situ hybridization to the region surrounding the Ewing's sarcoma breakpoint on chromosome 22. Three of these were initially ordered by pair-wise hybridization to metaphase chromosomes with differential detection of the probes. These and the remaining probes were then ordered by hybridizing two or threeprobes simultaneously to interphase nuclei. In the two probe experiments and some of the three probe experiments, the order was derived by comparing mean interphase distances between signals from the probes. In the three probe experiments, either two probes were detected with one fluorochrome and the third with another or all three probes were individually distinguished by detecting one probe with fluorescein isothiocyanate (FITC), one with Texas Red, and one with both fluorochromes to give a mixed color. The order of the signals was then noted. Greaterthan 60 percent of configurations with a discrete order were shown ordeduced to be correct. These approaches are assessed and we demonstrate a more obvious predominating order when all three probes are differentially detected. The order of the probes was deduced to be centromere: D22S271: D22S260: lambdaS1: D22S262: cosKI831: Ewing's sarcoma breakpoint: cosLIF: D22S261: lamdbaS15: telomere.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/07/20 alle ore 08:03:03