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Titolo:
NO EVIDENCE FOR QUASI-SPECIES POPULATIONS DURING PERSISTENCE OF THE CORONAVIRUS MOUSE HEPATITIS-VIRUS JHM - SEQUENCE CONSERVATION WITHIN THE SURFACE GLYCOPROTEIN GENE-S IN LEWIS RATS
Autore:
STUHLER A; FLORY E; WEGE H; LASSMANN H; WEGE H;
Indirizzi:
FED RES CTR VIRUS DIS ANIM,FRIEDRICH LOEFFLER INST,INST DIAGNOST VIROL D-17498 INSEL RIEMS GERMANY FED RES CTR VIRUS DIS ANIM,FRIEDRICH LOEFFLER INST,INST DIAGNOST VIROL D-17498 INSEL RIEMS GERMANY RES UNIT EXPT NEUROPATHOL A-1090 VIENNA AUSTRIA UNIV WURZBURG,INST VIROL D-97078 WURZBURG GERMANY
Titolo Testata:
Journal of General Virology
, volume: 78, anno: 1997,
parte:, 4
pagine: 747 - 756
SICI:
0022-1317(1997)78:<747:NEFQPD>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
INFECTIOUS-BRONCHITIS VIRUS; MURINE CORONAVIRUS; MEASLES-VIRUS; DEMYELINATING ENCEPHALOMYELITIS; MONOCLONAL-ANTIBODIES; PLASMID DNA; SSPE BRAIN; VARIANTS; RNA; NUCLEOTIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
A. Stuhler et al., "NO EVIDENCE FOR QUASI-SPECIES POPULATIONS DURING PERSISTENCE OF THE CORONAVIRUS MOUSE HEPATITIS-VIRUS JHM - SEQUENCE CONSERVATION WITHIN THE SURFACE GLYCOPROTEIN GENE-S IN LEWIS RATS", Journal of General Virology, 78, 1997, pp. 747-756

Abstract

The surface glycoprotein S (spike) of coronaviruses is believed to bean important determinant of virulence and displays extensive genetic polymorphism in cell culture isolates. This led us to consider whetherthe observed heterogeneity is reflected by a quasispecies distribution of mutated RNA molecules within the infected organ. Coronavirus infection of rodents is a useful model system for investigating the pathogenesis of virus-induced central nervous system (CNS) disease. Here, weinvestigated whether genetic changes in the S gene occurred during virus persistence in vivo. We analysed the variability of S gene sequences directly from the brain tissue of Lewis rats infected with the coronavirus mouse hepatitis virus (MHV) variant JHM-Pi using RT-PCR amplification methods. The S gene sequence displayed a remarkable genetic stability in vivo. No evidence for a quasispecies distribution was foundby sequence analysis of amplified S gene fragments derived from the CNS of Lewis rats. Furthermore, the S gene also remained conserved under the selection pressure of a neutralizing antibody. Only a few mutations predicted to result in amino acid changes were detected in single clones. The changes were not represented in the consensus sequence. These results indicate that to retain functional proteins under the constraints of a persistent infection in vivo, conservation of sequence can be more important than heterogeneity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 19:33:15