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Titolo:
POPULATION STUDY OF T-CELL RECEPTOR-V-BETA GENE USAGE IN PERIPHERAL-BLOOD LYMPHOCYTES - DIFFERENCES IN ETHNIC-GROUPS
Autore:
GEURSEN A; SKINNER MA; TOWNSEND LA; PERKO LK; FARMILOE SJ; PEAKE JS; SIMPSON IJ; FRASER JD; TAN PLJ;
Indirizzi:
UNIV AUCKLAND,SCH MED,DEPT MOLEC MED,PRIVATE BAG 92019 AUCKLAND NEW ZEALAND UNIV AUCKLAND,SCH MED,DEPT MOLEC MED,PRIVATE BAG 92019 AUCKLAND NEW ZEALAND
Titolo Testata:
Clinical and experimental immunology
fascicolo: 1, volume: 94, anno: 1993,
pagine: 201 - 207
SICI:
0009-9104(1993)94:1<201:PSOTRG>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; VARIABLE REGION GENES; ANTIGEN RECEPTOR; RHEUMATOID-ARTHRITIS; AUTOIMMUNE-DISEASES; REPERTOIRE; VARIABILITY; PREVALENCE; DIVERSITY; PANEL;
Keywords:
T-CELL RECEPTOR; TCR-V-BETA; GENE USAGE; POLYNESIAN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
A. Geursen et al., "POPULATION STUDY OF T-CELL RECEPTOR-V-BETA GENE USAGE IN PERIPHERAL-BLOOD LYMPHOCYTES - DIFFERENCES IN ETHNIC-GROUPS", Clinical and experimental immunology, 94(1), 1993, pp. 201-207

Abstract

The T cell receptor (TCR) Vbeta repertoire in peripheral blood lymphocytes (PBL) of a large number of healthy individuals was analysed by quantifying Vbeta-specific mRNA using the method of anchored multiprimer DNA amplification and a reverse dot blot assay. Among 16 Vbeta gene families examined, particular Vbeta genes were noted to be unequally expressed in the PBL of 70 healthy donors. The frequently used genes belong to the Vbeta 4, 5, 6, 8 and 13 (12) families, while Vbeta 1, 9 and 15 were the least frequently used gene families. This bias in gene usage was observed in all individuals. Marked deviation from the mean percentage usage was noted for some V# genes in individuals when their PBL were examined serially, but the common pattern of biased usage wasnot grossly distorted. When the TCR repertoire of different ethnic groups was examined, a lower mean frequency of Vbeta 3.2 was seen in therepertoire of 19 Caucasians compared with 25 age-matched Samoans (P<0.003). Conversely, the expression of Vbeta 5.1 and of Vbeta 5.3 was higher in Caucasians than in 51 age-matched Polynesians (Maoris and Samoans, P < 0.003). Considering the 20% co-efficient of variation in the estimate of Vbeta gene usage, our data from 70 unrelated individuals suggest that in PBL, individual variations in the TCR repertoire were superimposed upon a common biased usage of Vbeta genes in the general population.

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Documento generato il 27/01/20 alle ore 16:44:54