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Titolo:
ESTIMATES OF LINKAGE DISEQUILIBRIUM AND THE RECOMBINATION PARAMETER DETERMINED FROM SEGREGATING NUCLEOTIDE SITES IN THE ALCOHOL-DEHYDROGENASE REGION OF DROSOPHILA-PSEUDOOBSCURA
Autore:
SCHAEFFER SW; MILLER EL;
Indirizzi:
PENN STATE UNIV,DEPT BIOL UNIV PK PA 16802 PENN STATE UNIV,INST MOLEC EVOLUTIONARY GENET UNIV PK PA 16802
Titolo Testata:
Genetics
fascicolo: 2, volume: 135, anno: 1993,
pagine: 541 - 552
SICI:
0016-6731(1993)135:2<541:EOLDAT>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOLECULAR GENETIC-VARIATION; NEUTRAL ALLELE MODEL; NATURAL-POPULATIONS; SEQUENCE ANALYSIS; INTRAGENIC RECOMBINATION; ANANASSAE POPULATIONS; SAMPLING DISTRIBUTION; CENTROMERIC REGION; STATISTICAL TESTS; DNA POLYMORPHISM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
S.W. Schaeffer e E.L. Miller, "ESTIMATES OF LINKAGE DISEQUILIBRIUM AND THE RECOMBINATION PARAMETER DETERMINED FROM SEGREGATING NUCLEOTIDE SITES IN THE ALCOHOL-DEHYDROGENASE REGION OF DROSOPHILA-PSEUDOOBSCURA", Genetics, 135(2), 1993, pp. 541-552

Abstract

The alcohol dehydrogenase (Adh) region of Drosophila pseudoobscura, which includes the two genes Adh and Adh-Dup, was used to examine the pattern and organization of linkage disequilibrium among pairs of segregating nucleotide sites. A collection of 99 strains from the geographic range of D. pseudoobscura were nucleotide-sequenced with polymerase chain reaction-mediated techniques. All pairs of the 359 polymorphic sites in the 3.5-kb Adh region were tested for significant linkage disequilibrium with Fisher's exact test. Of the 74,278 pairwise comparisons of segregating sites, 127 were in significant linkage disequilibriumat the 5% level. The distribution of five linkage disequilibrium estimators D(ij), D 2, r(ij), r2 and D(ij) were compared to theoretical distributions. The observed distributions of D(ij), D2, r(ij) and r2 were consistent with the theoretical distribution given an infinite sitesmodel. The observed distribution of D(ij) differed from the theoretical distribution because of an excess of values at -1 and 1. No spatialpattern was observed in the linkage disequilibrium pattern in the Adhregion except for two clusters of sites nonrandomly associated in theadult intron and intron 2 of Adh. The magnitude of linkage disequilibrium decreases significantly as nucleotide distance increases, or a distance effect. Adh-Dup had a larger estimate of the recombination parameter, 4Nc, than Adh, where N is the effective population size and c is the recombination rate. A comparison of the mutation and recombination parameters shows that 7-17 recombination events occur for each mutation event. The heterogeneous estimates of the recombination parameterand the inverse relationship between linkage disequilibrium and nucleotide distance are no longer significant when the two clusters of Adh intron sites are excluded from analyses. The most likely explanation for the two clusters of linkage disequilibria is epistatic selection between sites in the cluster to maintain pre-mRNA secondary structure.

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Documento generato il 02/12/20 alle ore 16:57:05