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Titolo:
CHARACTERIZATION OF EXPRESSION OF A MITOCHONDRIAL GENE REGION ASSOCIATED WITH THE BRASSICA-POLIMA CMS - DEVELOPMENTAL INFLUENCES
Autore:
SINGH M; BROWN GG;
Indirizzi:
MCGILL UNIV,DEPT BIOL MONTREAL H3A 1B1 QUEBEC CANADA MCGILL UNIV,DEPT BIOL MONTREAL H3A 1B1 QUEBEC CANADA
Titolo Testata:
Current genetics
fascicolo: 4, volume: 24, anno: 1993,
pagine: 316 - 322
SICI:
0172-8083(1993)24:4<316:COEOAM>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOPLASMIC MALE-STERILITY; TRANSCRIPTION INITIATION SITES; OXIDASE SUBUNIT-I; MALE-FERTILITY; RIBOSOMAL-RNA; NAPUS L; SEQUENCE; MAIZE; PLANTS; IDENTIFICATION;
Keywords:
CYTOPLASMIC MALE STERILITY; BRASSICA; MITOCHONDRIAL DNA; GENE EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
M. Singh e G.G. Brown, "CHARACTERIZATION OF EXPRESSION OF A MITOCHONDRIAL GENE REGION ASSOCIATED WITH THE BRASSICA-POLIMA CMS - DEVELOPMENTAL INFLUENCES", Current genetics, 24(4), 1993, pp. 316-322

Abstract

The mitochondrial genome of the Polima (pol) male-sterile cytoplasm of Brassica napus contains a chimeric 224-codon open reading frame (orf224) that is located upstream of, and co-transcribed with, the atp6 gene. The N-terminal coding region of orf224 is derived from a conventional mitochondrial gene, orfB, while the origin of the remainder of thesequence is unknown. We show that an apparently functional copy of orfB is present in the pol mitochondrial genome, indicating that the polCMS is not caused by the absence of an intact, expressed orfB gene. The 5' termini of orf224/atp6 transcripts present in both sterile and fertility-restored (Rf) pol cytoplasm plants are shown to map to sequences resembling mitochondrial transcription-initiation sites, whereas the 5' termini of two transcripts specific to restored lines map to sequences which resemble neither one another nor mitochondrial promoter motifs. It is suggested that the complex orf224/atp6 transcript patternof Rf plants is generated by a combination of multiple transcription initiation and processing events and that the nuclear restorer gene acts to specifically alter orf224/atp6 transcripts by affecting RNA processing. Northern analyses demonstrate that the effect of the restorer gene on orf224/atp6 transcripts is not tissue or developmental-stage specific. However, the expression of the atp6 region is developmentallyregulated in pol plants, resulting in decreased levels of monocistronic atp6 transcripts in floral tissue relative to seedlings. It is suggested that this developmental regulation may be related to the absenceof overt phenotypic effects of the CMS mutation in vegetative tissues.

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Documento generato il 29/09/20 alle ore 06:48:27