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Titolo:
IDENTIFICATION OF A REL-RELATED PROTEIN IN THE NUCLEUS DURING THE S-PHASE OF THE CELL-CYCLE
Autore:
EVANS RB; GOTTLIEB PD; BOSE HR;
Indirizzi:
UNIV TEXAS,DEPT MICROBIOL AUSTIN TX 78712 UNIV TEXAS,DEPT MICROBIOL AUSTIN TX 78712 UNIV TEXAS,CELL RES INST AUSTIN TX 78712
Titolo Testata:
Molecular and cellular biology
fascicolo: 10, volume: 13, anno: 1993,
pagine: 6147 - 6156
SICI:
0270-7306(1993)13:10<6147:IOARPI>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
NF-KAPPA-B; SODIUM DODECYL-SULFATE; TRANSFORMED LYMPHOID-CELLS; DNA-BINDING SUBUNIT; C-REL; V-REL; PROTOONCOGENE PRODUCT; MOUSE FIBROBLASTS; G0-TO-G1 TRANSITION; GEL-ELECTROPHORESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
62
Recensione:
Indirizzi per estratti:
Citazione:
R.B. Evans et al., "IDENTIFICATION OF A REL-RELATED PROTEIN IN THE NUCLEUS DURING THE S-PHASE OF THE CELL-CYCLE", Molecular and cellular biology, 13(10), 1993, pp. 6147-6156

Abstract

The c-rel proto-oncogene encodes a 75-kDa protein (p75c-rel) which ispresent in the cytosol of chick embryo fibroblasts (CEF) associated with a distinct set of cellular proteins with molecular masses of 40, 115, and 124 kDa. CEF cultures arrested in S phase of the cell cycle, or enriched for G2 or mitotic cells, were examined to determine whetherthe expression of c-rel was altered during the cell cycle. Levels of p75c-rel remained constant in all portions of the cell cycle examined;however, a Rel-related protein with an apparent molecular mass of 64 kDa was detected in nuclei of S-phase cells. As cells enter G2, the level of this protein in the nucleus decreases. This protein reacts withantiserum generated against the carboxy terminus of p75c-rel in radioimmunoprecipitations and Western immunoblot experiments and was also detected in a Western immunoblot with antiserum generated against the first 161 amino acids of pp59v-rel. Thus, unlike other Rel/NF-kappaB family members, p64 has carboxy-terminal homology with c-Rel. The majority of peptides generated by partial proteolytic cleavage of p64 are shared with peptides generated by digestion of p75c-rel and/or pp59v-rel. We suggest that this protein represents a new member of the Rel family of transcription factors and is located in the nucleus of avian fibroblasts during S phase of the cell cycle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 12:07:46