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Titolo:
TNF-ALPHA INDUCES EXPRESSION OF THE CHEMOATTRACTANT CYTOKINE RANTES IN CULTURED MOUSE MESANGIAL CELLS
Autore:
WOLF G; ABERLE S; THAISS F; NELSON PJ; KRENSKY AM; NEILSON EG; STAHL RAK;
Indirizzi:
UNIV HAMBURG,DEPT MED,DIV NEPHROL,MARTINSTR 52 D-20251 HAMBURG GERMANY UNIV FRANKFURT,DEPT MED,DIV NEPHROL W-6000 FRANKFURT GERMANY STANFORD UNIV,MED CTR,SCH MED,SCH MED,DEPT PEDIAT STANFORD CA 94305 UNIV PENN,DEPT MED,DIV RENAL ELECTROLYTE PHILADELPHIA PA 19104
Titolo Testata:
Kidney international
fascicolo: 4, volume: 44, anno: 1993,
pagine: 795 - 804
SICI:
0085-2538(1993)44:4<795:TIEOTC>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLOMERULAR INJURY; MURINE RANTES; RAT; PROLIFERATION; PEPTIDE-1; INTERLEUKIN-8; BIOSYNTHESIS; NEUTROPHILS; STIMULATION; MACROPHAGE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
G. Wolf et al., "TNF-ALPHA INDUCES EXPRESSION OF THE CHEMOATTRACTANT CYTOKINE RANTES IN CULTURED MOUSE MESANGIAL CELLS", Kidney international, 44(4), 1993, pp. 795-804

Abstract

We investigated the effect of several immune-relevant cytokines on expression of the chemoattractant intercrine/chemokine RANTES in a mousemesangial cell line (MMC). Fifty ng/ml recombinant tumor necrosis factor alpha (TNFalpha) induced a marked increase in RANTES transcripts after two hours. RANTES mRNA remained elevated for 24 to 48 hours afterstimulation, and could be abolished by co-incubation with 30 mug/ml of a neutralizing rabbit anti-TNFalpha antibody. Protein expression of RANTES, as assessed by indirect immunofluorescence and Western blotting, increased in MMCs 24 hours after TNFalpha stimulation. Interleukin-1beta, tumor necrosis factor beta (TNFbeta), and lipopolysaccharide (LPS) also increased expression of RANTES mRNA. In addition, RANTES mRNAexpression was stimulated in glomeruli harvested from rats following renal in vivo perfusion with TNFalpha. Our results indicate that mesangial cells produce the small cytokine RANTES. This factor, in concert with other chemoattractants, may play a role in the glomerular recruitment of inflammatory cells like macrophages/monocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 22:15:27