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Titolo:
MAPPING OF ANTIGENIC SITES OF COXSACKIEVIRUS-B3 BY SYNTHETIC PEPTIDES
Autore:
AUVINEN P; MAKELA MJ; ROIVAINEN M; KALLAJOKI M; VAINIONPAA R; HYYPIA T;
Indirizzi:
EUROPEAN MOLEC BIOL LAB,POSTFACH 102209 W-6912 HEIDELBERG GERMANY UNIV TURKU,DEPT VIROL SF-20500 TURKU 50 FINLAND NATL PUBL HLTH INST,DEPT VIROL,ENTEROVIRUS LAB HELSINKI FINLAND UNIV TURKU,DEPT PATHOL SF-20500 TURKU 50 FINLAND
Titolo Testata:
APMIS. Acta pathologica, microbiologica et immunologica Scandinavica
fascicolo: 7, volume: 101, anno: 1993,
pagine: 517 - 528
SICI:
0903-4641(1993)101:7<517:MOASOC>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMPLETE NUCLEOTIDE-SEQUENCE; NEUTRALIZING ANTIBODIES; POLIOVIRUS TYPE-1; MONOCLONAL-ANTIBODIES; CAPSID PROTEINS; VIRUS B3; INDUCTION; GENOME; RNA; PICORNAVIRUSES;
Keywords:
COXSACKIEVIRUS-B3; SYNTHETIC PEPTIDE; ANTIGENIC SITES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
P. Auvinen et al., "MAPPING OF ANTIGENIC SITES OF COXSACKIEVIRUS-B3 BY SYNTHETIC PEPTIDES", APMIS. Acta pathologica, microbiologica et immunologica Scandinavica, 101(7), 1993, pp. 517-528

Abstract

Peptides presenting predicted antigenic sites of CBV3 capsid proteinsand peptide sequences from conserved regions of the nonstructural proteins were synthesized, and rabbit antipeptide sera were tested for their immunoreactivity. Peptides derived from different capsid regions were able to induce production of neutralizing antibodies in rabbits. As measured by EIA, all peptides representing four different proposed antigenic sites were immunogenic, inducing an antibody response againstthe homologous peptide and purified CBV3 as measured by EIA. Immunization with inactivated CBV3 induced a secondary response especially in rabbits primed with peptides representing polypeptide VP2. Antisera against the nonstructural protein sequences were highly cross-reactive with other enteroviruses, while the capsid peptide antisera were mainlytype-specific when tested by immunoblotting against a panel of enteroviruses. Four of the capsid region peptides also exhibited distinct T-cell reactivity in a mouse T-cell proliferation assay.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 21:44:05