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Titolo:
ADP ATP TRANSLOCATOR FROM PEA ROOT PLASTIDS - COMPARISON WITH TRANSLOCATORS FROM SPINACH-CHLOROPLASTS AND PEA LEAF MITOCHONDRIA/
Autore:
SCHUNEMANN D; BORCHERT S; FLUGGE UI; HELDT HW;
Indirizzi:
INST BIOCHEM PFLANZE,UNTERE KARSPULE 2 D-37073 GOTTINGEN GERMANY INST BIOCHEM PFLANZE,UNTERE KARSPULE 2 D-37073 GOTTINGEN GERMANY JULIUS VON SACHS INST BIOWISSENSCH,LEHRSTUHL BOT 1 D-97082 WURZBURG GERMANY
Titolo Testata:
Plant physiology
fascicolo: 1, volume: 103, anno: 1993,
pagine: 131 - 137
SICI:
0032-0889(1993)103:1<131:AATFPR>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADENYLATE TRANSLOCATOR; PHOSPHATE TRANSLOCATOR; ATP/ADP-TRANSLOCATOR; METABOLITE TRANSPORT; STARCH BIOSYNTHESIS; BONGKREKIC ACID; OUTER-MEMBRANE; ENVELOPE; AMYLOPLASTS; POTATO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
D. Schunemann et al., "ADP ATP TRANSLOCATOR FROM PEA ROOT PLASTIDS - COMPARISON WITH TRANSLOCATORS FROM SPINACH-CHLOROPLASTS AND PEA LEAF MITOCHONDRIA/", Plant physiology, 103(1), 1993, pp. 131-137

Abstract

The kinetic properties of the adenosine 5'-diphosphate/adenosine 5'-triphosphate (ADP/ATP) translocator from pea (Pisum sativum L.) root plastids were determined by silicone oil filtering centrifugation and compared with those of spinach (Spinacia oleracea L.) chloroplasts and pea leaf mitochondria. In addition, the ADP/ATP transporting activitiesfrom the above organelles were reconstituted into liposomes. The K(m)(ATP) value of the pea root ADP/ATP translocator was 10 mum and that for ADP was 46 mum. Corresponding values of the spinach ADP/ATP translocator were 25 mum and 28 mum, respectively. Comparable results were obtained for the reconstituted ATP transport activities. The transport was highly specific for ATP and ADP. Adenosine 5'-monophosphate (AMP) caused only a slight inhibition and phosphoenolpyruvate and inorganic pyrophosphate caused no inhibition of ATP uptake. With pea root plastids and spinach chloroplasts, K(m) values >1 mM were obtained for ADP-glucose. Since the concentrations of ATP and ADP-glucose in the cytosolic compartment of spinach leaves have been determined as 2.5 and 0.6 mm, respectively, a transport of ADP-glucose by the ADP/ATP translocatordoes not appear to have any physiological significance in vivo. Although both the plastidial and the mitochondrial ADP/ATP translocators were inhibited to some extent by carboxyatractyloside, no immunological cross-reactivity was detected between the plastidial and the mitochondrial proteins. It seems probable that these proteins derive from different ancestors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 12:07:24