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Titolo:
OPIOID-GLUTAMATE INTERACTIONS IN RAT LOCUS-CERULEUS NEURONS
Autore:
OLESKEVICH S; CLEMENTS JD; WILLIAMS JT;
Indirizzi:
OREGON HLTH SCI UNIV,VOLLUM INST,3181 SW SAM JACKSON PK RD PORTLAND OR 97201 OREGON HLTH SCI UNIV,VOLLUM INST,3181 SW SAM JACKSON PK RD PORTLAND OR 97201
Titolo Testata:
Journal of neurophysiology
fascicolo: 3, volume: 70, anno: 1993,
pagine: 931 - 937
SICI:
0022-3077(1993)70:3<931:OIIRLN>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; ACTIVATION; CURRENTS; INVITRO; OPIATE; INTERNEURONS; MODULATION; INHIBITION; RESPONSES; RECEPTORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
S. Oleskevich et al., "OPIOID-GLUTAMATE INTERACTIONS IN RAT LOCUS-CERULEUS NEURONS", Journal of neurophysiology, 70(3), 1993, pp. 931-937

Abstract

1. The effect of mu-opioids on the glutamate response was investigated in rat locus coeruleus (LC) neurons by intracellular recording in the brain slice preparation. Glutamate responses were evoked by bath application of selective glutamate agonists, glutamate iontophoresis, andstimulation of excitatory afferents. 2. The mu-opioid agonist D-Ala2-MePhe4-Gly-ol5-enkephalin (DAMGO; 1 muM) potentiated the response to bath application of N-methyl-D-aspartate (NMDA) and alpha-amino-3-hydroxy-5-methyl-4-isoxazole proprionic acid by 91 and 142%, respectively, in slices cut in the horizontal plane. The mechanism of action of thiseffect was investigated under conditions that limited the DAMGO-induced hyperpolarization and improved the space clamp of the neuron through 1) addition of barium, 2) increase in extracellular potassium concentration, 3) sectioning of the LC in the coronal plane, and 4) additionof carbenoxolone. Each experimental manipulation decreased the DAMGO outward current and reduced the mu-opioid potentiation of the glutamate response. The results suggest that the mu-opioid-mediated potentiation of the glutamate response is dependent on membrane hyperpolarization. 3. Neither forskolin nor the phorbol ester 4b-phorbol 12,13-dibutyrate (PDBu) altered the glutamate-mediated inward currents. The potentiation of the glutamate response by DAMGO was not affected by PDBu. 4. The mu-opioids DAMGO and [met]5enkephalin (10 muM) did not significantly affect the NMDA receptor-mediated depolarization (mean 14%) evoked by local application of glutamate but inhibited the NMDA receptor-mediated synaptic potential (mean 25%). 5. In summary, DAMGO potentiated currents produced by bath-applied glutamate agonists that may result from poor voltage clamp control of the dendrites of LC neurons. Many of the above results could be approximated by a simple passive electricalmodel of an LC neuron.

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Documento generato il 21/09/20 alle ore 08:49:26