Catalogo Articoli (Spogli Riviste)
OPAC HELP
Titolo: THE SUBSTRATE-DEPLETION ERROR ON THE MULTIPLE MICHAELIS-MENTEN EQUATION WITH AND WITHOUT AN ADDED TERM LINEARLY DEPENDENT ON THE SUBSTRATE CONCENTRATION
Autore: HEIRWEGH KPM; VERMEIR M; MOLENBERGHS G;
- Indirizzi:
- CATHOLIC UNIV LEUVEN,HEPATOL LAB,CAMPUS GASTHUISBERG,HERESTR 49 B-3000 LOUVAIN BELGIUM UNIV INSTELLING ANTWERP,DEPT MATH & COMP SCI B-2610 WILRIJK BELGIUM
- Titolo Testata:
- Journal of biochemical and biophysical methods
fascicolo: 2,
volume: 27,
anno: 1993,
pagine: 151 - 156
- SICI:
- 0165-022X(1993)27:2<151:TSEOTM>2.0.ZU;2-Z
- Fonte:
- ISI
- Lingua:
- ENG
- Soggetto:
- AMPHIPATHIC LIGANDS; BINDING; ENZYME; PROTEIN;
- Keywords:
- SUBSTRATE-DEPLETION ERROR; ENZYMATIC CONVERSION; TRANSPORT KINETICS; EQUILIBRIUM BINDING; RECEPTOR BINDING;
- Tipo documento:
- Note
- Natura:
- Periodico
- Settore Disciplinare:
- Science Citation Index Expanded
- Science Citation Index Expanded
- Science Citation Index Expanded
- Citazioni:
- 13
- Recensione:
- Indirizzi per estratti:
-
-
-
- Citazione:
- K.P.M. Heirwegh et al., "THE SUBSTRATE-DEPLETION ERROR ON THE MULTIPLE MICHAELIS-MENTEN EQUATION WITH AND WITHOUT AN ADDED TERM LINEARLY DEPENDENT ON THE SUBSTRATE CONCENTRATION", Journal of biochemical and biophysical methods, 27(2), 1993, pp. 151-156
Abstract
Saturation curves for substrate interaction with an acceptor (enzyme,binding or carrier protein) are often analysed on the assumption thatthe amount of acceptor-bound substrate is negligible compared to its total amount. Analytical criteria permitting one to decide whether theassumption is justified or not for systems described by a single Michaelis-Menten equation have been derived previously. For more complicated systems, error formulae often cannot be obtained in closed form and, if obtainable, are unwieldy. How this more complicated problem can be tackled is shown for mixtures of acceptor sites described by a sum of several Michaelis-Menten terms, without or with an additional tem for 'non-specific' uptake or binding. In particular, it is shown that the maximum error, for which simple analytical expressions are obtained,provides a valid criterion for assessing whether substrate depletion is negligible or not.
ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/01/21 alle ore 17:47:45