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Titolo:
A RISK ASSESSMENT STUDY OF PLANT GENETIC-TRANSFORMATION USING AGROBACTERIUM AND IMPLICATIONS FOR ANALYSIS OF TRANSGENIC PLANTS
Autore:
BARRETT C; COBB E; MCNICOL R; LYON G;
Indirizzi:
TEAGASC,KINSEALY RES & DEV CTR,MALAHIDE RD DUBLIN 17 IRELAND SCOTTISH CROP RES INST DUNDEE DD2 5DA SCOTLAND
Titolo Testata:
Plant cell, tissue and organ culture
fascicolo: 2, volume: 47, anno: 1996,
pagine: 135 - 144
SICI:
0167-6857(1996)47:2<135:ARASOP>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
COAT PROTEIN GENE; CUCUMIS-MELO L; MEDIATED TRANSFORMATION; HIGH-FREQUENCY; DNA TRANSFER; SUGAR-BEET; TUMEFACIENS; BACTERIA; ELIMINATION; PSEUDOMONAS;
Keywords:
BINARY VECTOR; MINIMUM BACTERICIDAL CONCENTRATION; RESIDUAL AGROBACTERIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
C. Barrett et al., "A RISK ASSESSMENT STUDY OF PLANT GENETIC-TRANSFORMATION USING AGROBACTERIUM AND IMPLICATIONS FOR ANALYSIS OF TRANSGENIC PLANTS", Plant cell, tissue and organ culture, 47(2), 1996, pp. 135-144

Abstract

Agrobacterium transformation systems for Brassica, Solanum and Rubus,using carbenicillin, cefotaxime and ticaracillin respectively to eliminate contamination, were examined for the presence of residual Agrobacterium. The results indicated that none of the antibiotics in question, succeeded in eliminating Agrobacterium and the contamination levelsincreased in explants from 12 to 16 weeks to such an extent that Solanum cultures senesced and died. This may be due to the fact that four times the Minimum bactericidal concentration values (concentration to be used for elimination of contaminants in culture), for the three antibiotics, were higher than the concentrations employed in the culture medium. Contamination in shoot material decreased over 16 to 24 weeks possibly due to bacteriostatis and the use only of the apical node forfurther culture. The presence of the binary vector was also noted under non-selective conditions, even up to 6 months after transformation,where approx. 50% of contaminated material still harboured bacterial cells with the binary vector at levels of approx. 10(7) Colony formingunits per gram.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 10:55:01