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Titolo:
MEDIATION OF ENTRY OF HUMAN IMMUNODEFICIENCY VIRUS-1 INTO ALVEOLAR MACROPHAGES BY CD4 WITHOUT FACILITATION BY SURFACTANT-ASSOCIATED PROTEIN-A
Autore:
GUAY LA; SIERRAMADERO JG; FINEGAN CK; RICH EA;
Indirizzi:
CASE WESTERN RESERVE UNIV,DEPT MED,10900 EUCLID AVE CLEVELAND OH 44106 CASE WESTERN RESERVE UNIV,DEPT MED CLEVELAND OH 44106 UNIV HOSP CLEVELAND CLEVELAND OH 44106
Titolo Testata:
American journal of respiratory cell and molecular biology
fascicolo: 4, volume: 16, anno: 1997,
pagine: 421 - 428
SICI:
1044-1549(1997)16:4<421:MOEOHI>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECOMBINANT SOLUBLE CD4; MONONUCLEAR PHAGOCYTES; HUMAN-MONOCYTES; HIV-INFECTION; T-CELLS; MONOCLONAL-ANTIBODIES; INVITRO INFECTION; FC RECEPTOR; II CELLS; TYPE-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
L.A. Guay et al., "MEDIATION OF ENTRY OF HUMAN IMMUNODEFICIENCY VIRUS-1 INTO ALVEOLAR MACROPHAGES BY CD4 WITHOUT FACILITATION BY SURFACTANT-ASSOCIATED PROTEIN-A", American journal of respiratory cell and molecular biology, 16(4), 1997, pp. 421-428

Abstract

The mechanism of uptake of human immunodeficiency virus-1 (HIV-1) into alveolar macrophages (AM), freshly isolated blood monocytes (MN), and cultured MN (CM) was investigated focusing on the role of CD4 and ofsurfactant-associated protein A (SP-A). By radioimmunoassay which obviated the problems of auto- and nonspecific fluorescence of more differentiated macrophages, each of the macrophage populations studied expressed CD4. Semiquantitative polymerase chain reaction was performed toassess uptake of HIV-1(JR-FL) into cells. OKT4a (directed against CD4) blocked uptake of HIV-1 into CM, AM, and MN by 67 to 100%. OKT4 (directed against another epitope of CD4) had a smaller and less consistent effect (0-90%), and control antibodies showed minimal effects and only at supersaturating concentrations. SP-A had no effect on uptake of HIV-1 into AM. SP-A also had no consistent effect on production of HIV-1(JR-FL) by AM infected in vitro (p24 antigen ELISA). Thus CD4 is themajor receptor for HIV-1 in mononuclear phagocytes, including AM, andSP-A does not modulate entry.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 11:01:50