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Titolo:
BIDIRECTIONAL TRANSCYTOSIS DETERMINES THE STEADY-STATE DISTRIBUTION OF THE TRANSFERRIN RECEPTOR AT OPPOSITE PLASMA-MEMBRANE DOMAINS OF BEWOCELLS
Autore:
CERNEUS DP; STROUS GJ; VANDERENDE A;
Indirizzi:
UNIV UTRECHT,SCH MED,DEPT CELL BIOL,AZU H02 314,HEIDELBERGLAAN 100 3584 CH UTRECHT NETHERLANDS UNIV UTRECHT,SCH MED,DEPT CELL BIOL,AZU H02 314,HEIDELBERGLAAN 100 3584 CH UTRECHT NETHERLANDS
Titolo Testata:
The Journal of cell biology
fascicolo: 6, volume: 122, anno: 1993,
pagine: 1223 - 1230
SICI:
0021-9525(1993)122:6<1223:BTDTSD>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERIC IMMUNOGLOBULIN RECEPTOR; CANINE KIDNEY-CELLS; CYTOPLASMIC DOMAIN; MDCK CELLS; PATHWAYS; PROTEINS; CACO-2; LOCALIZATION; ENDOCYTOSIS; EPITHELIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
D.P. Cerneus et al., "BIDIRECTIONAL TRANSCYTOSIS DETERMINES THE STEADY-STATE DISTRIBUTION OF THE TRANSFERRIN RECEPTOR AT OPPOSITE PLASMA-MEMBRANE DOMAINS OF BEWOCELLS", The Journal of cell biology, 122(6), 1993, pp. 1223-1230

Abstract

Trophoblast-like BeWo cells form well-polarized epithelial monolayers, when cultured on permeable supports. Contrary to other polarized cell systems, in which the transferrin receptor is found predominantly onthe basolateral cell surface, BeWo cells express the transferrin receptor at both apical and basolateral cell surfaces (Cerneus, D.P., and A. van der Ende. 1991. J. Cell Biol. 114: 1149-1158). In the present study we have addressed the question whether BeWo cells use a differentsorting mechanism to target transferrin receptors to the cell surface, by examining the biosynthetic and transcytotic pathways of the transferrin receptor in BeWo cells. Using trypsin and antibodies to detect transferrin receptors at the cell surface of filter-grown BeWo cells, we show that at least 80% of newly synthesized transferrin receptor follows a direct pathway to the basolateral surface, demonstrating that the transferrin receptor is efficiently intracellularly sorted. After surface arrival, pulse-labeled transferrin receptor equilibrates between apical and basolateral cell surfaces, due to ongoing transcytotic transport in both directions. The subsequent redistribution takes over 120 min and results in a steady state distribution with 1.5-2.0 times more transferrin receptors at the basolateral surface than at the apical surface. By monitoring the fate of surface-bound I-125-transferrin,internalized either from the apical or basolateral surface transcytosis of the transferrin receptor was studied. About 15% of I-125-transferrin is transcytosed in the basolateral to apical direction, whereas 25% is transcytosed in the opposite direction, indicated that the fraction of receptors involved in transcytosis is roughly twofold higher for the apical receptor pool, as compared to the basolateral pool. Upon internalization, both apical and basolateral receptor pools become redistributed on both surfaces, resulting in a twofold higher number of transferrin receptors at the basolateral surface. Our results indicate that in BeWo cells bidirectional transcytosis is the main factor in surface distribution of transferrin receptors on apical and basolateral surfaces, which may represent a cell type-specific, post-endocytic, sorting mechanism.

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Documento generato il 19/09/20 alle ore 14:12:10