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Titolo:
CRYOPRESERVATION OF PENAEID PRAWN EMBRYOS
Autore:
SUBRAMONIAM T; NEWTON SS;
Indirizzi:
UNIV MADRAS,DEPT ZOOL,GUINDY CAMPUS MADRAS 600025 TAMIL NADU INDIA
Titolo Testata:
Current Science
fascicolo: 2, volume: 65, anno: 1993,
pagine: 176 - 178
SICI:
0011-3891(1993)65:2<176:COPPE>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
VITRIFICATION;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
7
Recensione:
Indirizzi per estratti:
Citazione:
T. Subramoniam e S.S. Newton, "CRYOPRESERVATION OF PENAEID PRAWN EMBRYOS", Current Science, 65(2), 1993, pp. 176-178

Abstract

The cryopreservation of penaeid prawn embryos has not been successfully carried out so far although standard protocols for several mammalian embryos are available. Penaeid prawn embryos develop in the sea water medium and hence the methodology developed for mammalian embryos, whose development takes place in the intra-uterine environment, cannot be applied for prawn embryo cryopreservation. We have successfully frozen, for the first time, the nauplii of the penaeid prawn P. indicus to-30-degrees-C and -40-degrees-C using liquid nitrogen vapour. In order to develop the freezing protocol for these embryos we have considered the following principal factors: cryoprotectant toxicity, addition and dilution of cryoprotectant, equilibration time, freezing and thawing rates. The response of the morula stage embryos (2 h after spawning)and nauplii to several permeating cryoprotectants were studied in detail. The hatch percentage was used as an index to evaluate survival ofcryoprotectant-treated morulae and direct observation of morphology and motility was used to assess survival of cryoprotectant-treated as well as frozen nauplii. Our initial attempt to vitrify the embryos was not successful.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 15:36:59