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OPAC HELP

Titolo:
3-DIMENSIONAL STRUCTURE OF A HETEROCLITIC ANTIGEN-ANTIBODY CROSS-REACTION COMPLEX
Autore:
CHITARRA V; ALZARI PM; BENTLEY GA; BHAT TN; EISELE JL; HOUDUSSE A; LESCAR J; SOUCHON H; POLJAK RJ;
Indirizzi:
INST PASTEUR,UNITE IMMUNOL STRUCT,CNRS,URA 359,25 RUE DR ROUX F-75724PARIS 15 FRANCE INST PASTEUR,UNITE IMMUNOL STRUCT,CNRS,URA 359,25 RUE DR ROUX F-75724PARIS 15 FRANCE
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 16, volume: 90, anno: 1993,
pagine: 7711 - 7715
SICI:
0027-8424(1993)90:16<7711:3SOAHA>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
EGG-WHITE LYSOZYME; CRYSTAL-STRUCTURES; SPECIFICITY; FRAGMENTS; FV;
Keywords:
CRYSTAL STRUCTURE; HETEROCLITIC ANTIBODY; LYSOZYME;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
V. Chitarra et al., "3-DIMENSIONAL STRUCTURE OF A HETEROCLITIC ANTIGEN-ANTIBODY CROSS-REACTION COMPLEX", Proceedings of the National Academy of Sciences of the United Statesof America, 90(16), 1993, pp. 7711-7715

Abstract

Although antibodies are highly specific, cross-reactions are frequently observed. To understand the molecular basis of this phenomenon, we studied the anti-hen egg lysozyme (HEL) monoclonal antibody (mAb) D11.15, which cross-reacts with several avian lysozymes, in some cases with a higher affinity (heteroclitic binding) than for HEL. We have determined the crystal structure of the Fv fragment of D11.15 complexed with pheasant egg lysozyme (PHL). In addition, we have determined the structure of PHL, Guinea fowl egg lysozyme, and Japanese quail egg lysozyme. Differences in the affinity of D11.15 for the lysozymes appear to result from sequence substitutions in these antigens at the interface with the antibody. More generally, cross-reactivity is seen to requirea stereochemically permissive environment for the variant antigen residues at the antibody-antigen interface.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 19:19:42