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Titolo:
COMPARISON OF FLUORESCENT VOLTAGE-SENSITIVE DYES FOR MULTISITE OPTICAL-RECORDING IN HAMSTER CEREBRAL COLTER BY MEASUREMENT OF BICUCULLINE-INDUCED EPILEPTIFORM EVENTS
Autore:
ZEIGER JD;
Indirizzi:
UNIV CONNECTICUT,CTR HLTH,SCH DENT MED,DEPT BIOSTRUCT & FUNCT FARMINGTON CT 06030
Titolo Testata:
NeuroImage
fascicolo: 2, volume: 5, anno: 1997,
pagine: 154 - 163
SICI:
1053-8119(1997)5:2<154:COFVDF>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHARGE-SHIFT PROBES; ELECTRICAL-ACTIVITY; NEURONAL-ACTIVITY; VISUAL-CORTEX; RAPID CHANGES; MEMBRANE; MECHANISMS; SIGNALS; AXONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
J.D. Zeiger, "COMPARISON OF FLUORESCENT VOLTAGE-SENSITIVE DYES FOR MULTISITE OPTICAL-RECORDING IN HAMSTER CEREBRAL COLTER BY MEASUREMENT OF BICUCULLINE-INDUCED EPILEPTIFORM EVENTS", NeuroImage, 5(2), 1997, pp. 154-163

Abstract

Two fluorescent voltage-sensitive dyes, RH795 and DI-2-ANEPPQ, were compared for in vivo multisite optical recording from the gustatory insular cortex of the golden Syrian hamster, the first reported use of DI-2-ANEPPQ in a mammalian brain preparation. The exposed cortex of the anesthetized hamster was stained with a 500 mu M solution of either dye, and the cortical surface was imaged onto a 124-element photodiode array by an epifluorescence microscope equipped with a 2.5x objective (0.08 na) and appropriate excitation and emission filters for each dye. Background fluorescence was recorded with amplifiers in DC-coupled mode, and the bathing solution changed to one containing 100 mu M bicuculline methiodide. Large, widespread epileptiform events were recorded optically, and with a surface electrode, within 2 min. Three experiments were carried out with each dye. The 10 recorded events from each experiment (and five detector records from each of these 10) having the largest signals were selected for comparison. Five measures were derived from the data: (1) The ratio of peak signal fluorescence to background fluorescence (Delta F/F), (2) signal-to-noise power ratio (S/N), (3) root mean square noise (RMS noise), (4) an amplitude ratio (A(R)) consisting of the signal height divided by RMS noise, and (5) the peak value of the surface electrode record (SER). The results indicate a twofold increase in Delta F/F and a fivefold increase in S/N (twofold increase in A(R)) with DI-2-ANEPPQ. No significant difference was found between dyes in the RMS noise or SERs. In addition, signals did not decline detectably over time with DI-2-ANEPPQ, but declined about 25%/h with RH795. (C) 1997 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 22:11:44