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Titolo:
NOVEL VARIANTS OF CD44 ARISING FROM ALTERNATIVE SPLICING - CHANGES INTHE CD44 ALTERNATIVE SPLICING PATTERN OF MCF-7 BREAST-CARCINOMA CELLSTREATED WITH HYALURONIDASE
Autore:
TANABE KK; NISHI T; SAYA H;
Indirizzi:
MASSACHUSETTS GEN HOSP,DEPT SURG,DIV SURG ONCOL,FRUIT ST BOSTON MA 02114 UNIV TEXAS,MD ANDERSON CANC CTR,DEPT GEN SURG HOUSTON TX 77025 UNIV TEXAS,MD ANDERSON CANC CTR,DEPT NEUROONCOL HOUSTON TX 77025
Titolo Testata:
Molecular carcinogenesis
fascicolo: 4, volume: 7, anno: 1993,
pagine: 212 - 220
SICI:
0899-1987(1993)7:4<212:NVOCAF>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
LYMPHOCYTE HOMING RECEPTOR; MONOCLONAL-ANTIBODIES; HIGH ENDOTHELIUM; MOLECULAR-CLONING; DISTINCT; GLYCOPROTEIN; RECOGNITION; EXPRESSION; ACTIVATION; PGP-1;
Keywords:
ADHESION MOLECULE; RNA SPLICING; HYALURONATE; HYALURONIDASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
K.K. Tanabe et al., "NOVEL VARIANTS OF CD44 ARISING FROM ALTERNATIVE SPLICING - CHANGES INTHE CD44 ALTERNATIVE SPLICING PATTERN OF MCF-7 BREAST-CARCINOMA CELLSTREATED WITH HYALURONIDASE", Molecular carcinogenesis, 7(4), 1993, pp. 212-220

Abstract

CD44 is a cell-surface glycoprotein postulated to play a role in a variety of biological processes, including lymphocyte homing and tumor-cell metastasis. Several isoforms of CD44 have been identified in humancells, and the genesis of some of these isoforms has been attributed to alternative splicing. In the study presented here we amplified three novel transcript variants of CD44 from human cell lines using a reverse transcriptase-polymerase chain reaction strategy. Two of the novelisoforms differed from previously described CD44 isoforms as a resultof alternative splicing that occurred at previously reported splice junctions. The third novel CD44 isoform was generated from a previouslyunreported alternative splice junction near the 5' end of the open reading frame. Southern blot analysis of genomic DNA revealed that thesenovel isoforms and all of the previously described CD44 isoforms arose from alternative splicing. The capability of cells to modify their CD44 alternative splicing pattern was demonstrated in MCF-7 cells, which altered their CD44-isoform expression pattern in response to treatment with hyaluronidase. A better understanding of mechanisms regulatingCD44 alternative splicing may provide insights into diverse processes, including tumor-cell metastasis and lymphocyte homing. (C) 1993 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 14:19:40