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Titolo:
EVOLUTION AND REPLICATION OF TOBACCO RINGSPOT VIRUS SATELLITE RNA MUTANTS
Autore:
ROBAGLIA C; BRUENING G; HASELOFF J; GERLACH WL;
Indirizzi:
INRA,BIOL CELLULAIRE LAB F-78026 VERSAILLES FRANCE UNIV CALIF DAVIS,DEPT PLANT PATHOL DAVIS CA 95616 MASSACHUSETTS GEN HOSP,DEPT MOLEC BIOL BOSTON MA 02114 CSIRO,DIV PLANT IND CANBERRA ACT 2601 AUSTRALIA
Titolo Testata:
EMBO journal
fascicolo: 7, volume: 12, anno: 1993,
pagine: 2969 - 2976
SICI:
0261-4189(1993)12:7<2969:EAROTR>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
SELF-CLEAVAGE; RECOMBINATION; PLANTS; MODEL; GENE; DNA; COMPLEMENTARY; TRANSCRIPTS; RESISTANCE; DISEASE;
Keywords:
HAMMERHEAD RIBOZYME; MOLECULAR EVOLUTION; PLANT VIRUS; RNA CONFORMATION; SATELLITE RNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
C. Robaglia et al., "EVOLUTION AND REPLICATION OF TOBACCO RINGSPOT VIRUS SATELLITE RNA MUTANTS", EMBO journal, 12(7), 1993, pp. 2969-2976

Abstract

The replication properties of linker insertion-deletion mutants of tobacco ringspot virus satellite RNA have been studied by amplification in plants infected with the helper virus. Sequence analysis of the cDNAs corresponding to the replicated forms shows that only one of the original mutated molecules replicates unaltered, and in general new variants accumulate. Depending on the location of the original mutation three types of sequence modifications were observed: (i) deletion of themutated region followed by sequence duplication, (ii) sequence duplication and deletion outside of the mutated region and (iii) limited rearrangements at the site of mutation. The mutant that replicates without sequence changes accumulates linear multimeric forms suggesting thatself-cleavage is affected although the sequence alteration does not involve the hammerhead catalytic domain. Alternative RNA conformations are likely to play a role in the origin of this phenotype and in the formation of sequence duplications. These results demonstrate the greatstructural flexibility of this satellite RNA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 20:16:36