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Titolo:
KETTIN, A LARGE MODULAR PROTEIN IN THE Z-DISC OF INSECT MUSCLES
Autore:
LAKEY A; LABEIT S; GAUTEL M; FERGUSON C; BARLOW DP; LEONARD K; BULLARD B;
Indirizzi:
EUROPEAN MOLEC BIOL LAB,MEYERHOFSTR 1 W-6900 HEIDELBERG GERMANY EUROPEAN MOLEC BIOL LAB,MEYERHOFSTR 1 W-6900 HEIDELBERG GERMANY INST MOLEC PATHOL A-1030 VIENNA AUSTRIA
Titolo Testata:
EMBO journal
fascicolo: 7, volume: 12, anno: 1993,
pagine: 2863 - 2871
SICI:
0261-4189(1993)12:7<2863:KALMPI>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIGHT CHAIN KINASE; BEE FLIGHT-MUSCLE; MYOFIBRILLAR PROTEIN; ALPHA-ACTININ; Z-BAND; LEG MUSCLE; Z-LINES; TITIN; PURIFICATION; SEQUENCE;
Keywords:
CALPAIN; DROSOPHILA; INSECT MUSCLE; KETTIN; Z-DISC;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
A. Lakey et al., "KETTIN, A LARGE MODULAR PROTEIN IN THE Z-DISC OF INSECT MUSCLES", EMBO journal, 12(7), 1993, pp. 2863-2871

Abstract

Z-discs of insect flight muscle contain a large protein of 500-700 kDa. Monoclonal antibodies label an epitope in the molecule at the Z-disc in Drosophila and Lethocerus (waterbug). A partial cDNA of 1.6 kb from the Drosophila gene has been cloned and sequenced. The corresponding amino acid sequence has a modular structure composed of four conserved repeats of 95 amino acids homologous to immunoglobulin C2 domains (called class II domains in muscle proteins), separated by less conserved linker sequences of 35 amino acids. An expressed class II domain with flanking linker sequences binds to actin and alpha-actinin but not to myosin. Single molecules of the protein would be large enough to span the Z-disc. We suggest that the protein acts as scaffolding in the Z-disc and we call the protein kettin. The Ca2+ activated protease, calpain, disrupts the Z-disc of striated muscle, releasing alpha-actininintact. Calpain digests kettin to a series of peptides of between 30 and 170 kDa which are released from the myofibril. Digestion of kettinmay cause disintegration of the Z-disc and alpha-actinin release which lead to disassembly of the myofibril.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 15:56:42