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Titolo:
ENTEROPATHOGENIC ESCHERICHIA-COLI DECREASES THE TRANSEPITHELIAL ELECTRICAL-RESISTANCE OF POLARIZED EPITHELIAL MONOLAYERS
Autore:
CANIL C; ROSENSHINE I; RUSCHKOWSKI S; DONNENBERG MS; KAPER JB; FINLAY BB;
Indirizzi:
UNIV BRITISH COLUMBIA,BIOTECHNOL LAB VANCOUVER V6T 1Z3 BC CANADA UNIV BRITISH COLUMBIA,BIOTECHNOL LAB VANCOUVER V6T 1Z3 BC CANADA UNIV BRITISH COLUMBIA,DEPT BIOCHEM VANCOUVER V6T 1Z3 BC CANADA UNIV BRITISH COLUMBIA,DEPT MICROBIOL VANCOUVER V6T 1Z3 BC CANADA UNIV MARYLAND,SCH MED,CTR VACCINE DEV BALTIMORE MD 21201 UNIV MARYLAND,SCH MED,DEPT MED BALTIMORE MD 21201 UNIV MARYLAND,SCH MED,DIV INFECT DIS BALTIMORE MD 21201
Titolo Testata:
Infection and immunity
fascicolo: 7, volume: 61, anno: 1993,
pagine: 2755 - 2762
SICI:
0019-9567(1993)61:7<2755:EEDTTE>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
CLASSIC SEROTYPES; PLASMID; CELLS; CONSTRUCTION; SALMONELLA; ADHESION; DIARRHEA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
C. Canil et al., "ENTEROPATHOGENIC ESCHERICHIA-COLI DECREASES THE TRANSEPITHELIAL ELECTRICAL-RESISTANCE OF POLARIZED EPITHELIAL MONOLAYERS", Infection and immunity, 61(7), 1993, pp. 2755-2762

Abstract

The mechanisms whereby enteropathogenic Escherichia coli (EPEC) causes diarrhea remain undefined. We found that EPEC caused a decrease in transepithelial electrical resistance across polarized monolayers of Caco-2 and MDCK epithelial cells. This occurred approximately 6 to 10 h after bacterial addition and was reversible if the monolayers were treated with tetracycline or gentamicin. Although significant alterationsin host actin occurred beneath adherent EPEC, actin filaments supporting tight junctions were not noticeably affected in the epithelial cells, nor was the distribution of ZO-1, a tight junction protein. Despite the decrease in transepithelial electrical resistance, EPEC did not cause an increase in [H-3]inulin penetration across MDCK monolayers. Unlike in the parental strain, mutations in any loci involved in adherence or formation of attaching and effacing lesions were unable to cause a decrease in transepithelial resistance. These data indicate that EPEC causes a decrease in transepithelial electrical resistance by disrupting a transcellular (intracellular) pathway rather than by disrupting intercellular tight junctions (paracellular) and that these disruptions occur only when attaching and effacing lesions are formed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 05:26:11