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Titolo:
HUMAN SR PROTEINS AND ISOLATION OF A CDNA-ENCODING SRP75
Autore:
ZAHLER AM; NEUGEBAUER KM; STOLK JA; ROTH MB;
Indirizzi:
FRED HUTCHINSON CANC RES CTR,DIV BASIC SCI,A3-015,1124 COLUMBIA ST SEATTLE WA 98104 FRED HUTCHINSON CANC RES CTR,DIV BASIC SCI,A3-015,1124 COLUMBIA ST SEATTLE WA 98104
Titolo Testata:
Molecular and cellular biology
fascicolo: 7, volume: 13, anno: 1993,
pagine: 4023 - 4028
SICI:
0270-7306(1993)13:7<4023:HSPAIO>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA-BINDING PROTEINS; MESSENGER-RNA; DROSOPHILA ENCODES; SPLICING INVITRO; POLYMERASE-II; GENE; TRANSCRIPTION; EXPRESSION; REGULATORS; SIMILARITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
A.M. Zahler et al., "HUMAN SR PROTEINS AND ISOLATION OF A CDNA-ENCODING SRP75", Molecular and cellular biology, 13(7), 1993, pp. 4023-4028

Abstract

SR proteins are a family of proteins that have a common epitope recognized by a monoclonal antibody (MAb104) that binds active sites of polymerase II transcription. Four of the SR family members have been shown to restore activity to an otherwise splicing-deficient extract (S100extract). Here we show that two untested SR proteins, SRp20 and SRp75, can also complement the splicing-deficient extract. We isolated a cDNA encoding SRp75 and found that this protein, like other SR proteins,contains an N-terminal P-NA recognition motif (RRM), a glycine-rich region, an internal region homologous to the RRM, and a long (315-amino-acid) C-terminal domain composed predominantly of alternating serine and arginine residues. The apparent molecular mass of dephosphorylatedSRp75 is 57 kDa, the size predicted from the cDNA clone. We also detected mobility shifts after dephosphorylating SRp55, SRp40, SRp30a, andSRp30b; the sizes of the shifts are proportional to the length of theSR domain, suggesting that serines in this domain are phosphorylated.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 01:53:54