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Titolo:
WHOLE-CELL REPETITIVE ELEMENT SEQUENCE-BASED POLYMERASE CHAIN-REACTION ALLOWS RAPID ASSESSMENT OF CLONAL RELATIONSHIPS OF BACTERIAL ISOLATES
Autore:
WOODS CR; VERSALOVIC J; KOEUTH T; LUPSKI JR;
Indirizzi:
BAYLOR COLL MED,INST MOLEC GENET HOUSTON TX 77030 BAYLOR COLL MED,INST MOLEC GENET HOUSTON TX 77030 BAYLOR COLL MED,DEPT PEDIAT HOUSTON TX 77030
Titolo Testata:
Journal of clinical microbiology
fascicolo: 7, volume: 31, anno: 1993,
pagine: 1927 - 1931
SICI:
0095-1137(1993)31:7<1927:WRESPC>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
CITROBACTER-DIVERSUS; NEONATAL MENINGITIS; STRAINS; PLASMIDS; PCR;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
C.R. Woods et al., "WHOLE-CELL REPETITIVE ELEMENT SEQUENCE-BASED POLYMERASE CHAIN-REACTION ALLOWS RAPID ASSESSMENT OF CLONAL RELATIONSHIPS OF BACTERIAL ISOLATES", Journal of clinical microbiology, 31(7), 1993, pp. 1927-1931

Abstract

Repetitive element sequence-based polymerase chain reaction (rep-PCR)enables the generation of DNA fingerprints which discriminate bacterial species and strains. We describe the application of whole-cell methods which allow specimens from broth cultures or colonies from agar plates to be utilized directly in the PCR mixture. The rep-PCR-generatedDNA fingerprints obtained with whole-cell samples match results obtained with genomic DNA templates. Examples with different gram-negative bacteria (e.g., Citrobacter diversus, Escherichia coli, and Pseudomonas aeruginosa) and gram-positive bacteria (e.g., Staphylococcus aureus and Streptococcus pneumoniae) are demonstrated. Rapid specimen preparation methods enable rep-PCR-based fingerprinting to be completed in several hours and, therefore, allows the timely analysis of epidemiological relationships.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:41:08