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Titolo:
APPLICATION OF INDIRECT IMMUNOFLUORESCENCE TO DETECTION OF DIENTAMOEBA-FRAGILIS TROPHOZOITES IN FECAL SPECIMENS
Autore:
CHAN FTH; GUAN MX; MACKENZIE AMR;
Indirizzi:
CHILDRENS HOSP EASTERN ONTARIO,DEPT LAB MED OTTAWA K1H 8L1 ONTARIO CANADA OTTAWA CIVIC HOSP,DIV MICROBIOL OTTAWA K1Y 4E9 ONTARIO CANADA
Titolo Testata:
Journal of clinical microbiology
fascicolo: 7, volume: 31, anno: 1993,
pagine: 1710 - 1714
SICI:
0095-1137(1993)31:7<1710:AOIITD>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTESTINAL PARASITES; ENZYME-IMMUNOASSAY; GIARDIASIS; DIAGNOSIS; STOOL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
F.T.H. Chan et al., "APPLICATION OF INDIRECT IMMUNOFLUORESCENCE TO DETECTION OF DIENTAMOEBA-FRAGILIS TROPHOZOITES IN FECAL SPECIMENS", Journal of clinical microbiology, 31(7), 1993, pp. 1710-1714

Abstract

An indirect fluorescent-antibody (IFA) assay was carried out to examine for the presence of Dientamoeba fragilis trophozoites in preserved fecal specimens. Antiserum to D. fragilis trophozoites was raised in arabbit with a dixenic culture of D. fragilis (ATCC 30948) from the American type Culture Collection. After absorption with Klebsiella pneumoniae and Bacteroides vulgatus, the immune rabbit serum was used for examination by the IFA assay. A total of 155 clinical samples were tested: 42 with no parasites, 9 with D. fragilis, and 104 with other parasites. The IFA assay identified seven D. fragilis organisms. Two specimens with doubtful IFA assay readings showed very scanty amounts of D. fragilis trophozoites on stained smears. There were no false-positive IFA assay readings. The IFA assay appeared to be a promising method because of its speed in screening. The specificity of the IFA assay indicates that other diagnostic tests such as an enzyme-linked immunosorbent assay could be developed to identify D. fragilis antigens in fecal specimens.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/21 alle ore 07:34:55