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Titolo:
CORTICOTROPIN-RELEASING FACTOR UP-REGULATES ITS OWN RECEPTOR GENE-EXPRESSION IN CORTICOTROPIC ADENOMA CELLS IN-VITRO
Autore:
SAKAI Y; HORIBA N; SAKAI K; TOZAWA F; KUWAYAMA A; DEMURA H; SUDA T;
Indirizzi:
HIROSAKI UNIV,SCH MED,DEPT MED,DIV 3,5 ZAIFU CHO HIROSAKI AOMORI 036 JAPAN NAGOYA NATL HOSP,DEPT NEUROSURG NAGOYA AICHI 460 JAPAN TOKYO WOMENS MED COLL,INST CLIN ENDOCRINOL,DEPT MED TOKYO 162 JAPAN
Titolo Testata:
The Journal of clinical endocrinology and metabolism
fascicolo: 4, volume: 82, anno: 1997,
pagine: 1229 - 1234
SICI:
0021-972X(1997)82:4<1229:CFUIOR>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RIBONUCLEIC-ACID; FACTOR CRF RECEPTORS; RAT-BRAIN; PITUITARY CORTICOTROPHS; BETA-ENDORPHIN; HORMONE; GLUCOCORTICOIDS; CLONING; HEART; PROOPIOMELANOCORTIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
Y. Sakai et al., "CORTICOTROPIN-RELEASING FACTOR UP-REGULATES ITS OWN RECEPTOR GENE-EXPRESSION IN CORTICOTROPIC ADENOMA CELLS IN-VITRO", The Journal of clinical endocrinology and metabolism, 82(4), 1997, pp. 1229-1234

Abstract

To investigate the expression of CRF receptor (CRF-R) in human corticotropic adenoma (hCA) cells, we analyzed messenger RNA (mRNA) levels of type-1 GRF-R (CRF-R1). Adenomas were obtained from 10 patients with Gushing's disease. Northern blot analysis using a rat GRF-R1 complementary RNA probe revealed a main hybridization band of 2.7 kilobases in all the hCAs. The GRF-R1 mRNA level significantly increased after 1 h,reached 15-fold the basal level at 8 h, and remained elevated 24 h after the addition of 10 nmol/L GRF in vitro. Dose dependency of the stimulatory effect of CRF was also demonstrated in hCA cells, whereas CRFdown-regulated CRF-R1 mRNA levels in rat anterior pituitary (AP) cells. Treatment with dexamethasone or vasopressin decreased the CRF-R1 mRNA level in hCA cells, as observed in rat AP cells. In conclusion, we detected CRF-R1 mRNA in all hCAs tested. The GRF-R1 mRNA level was up-regulated by GRF itself in cultured hCA cells, in contrast to the down-regulation in rat AP cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 01:01:55