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Titolo:
KINETICS OF P56(LCK) AND P60(SRC) SRC HOMOLOGY-2 DOMAIN BINDING TO TYROSINE-PHOSPHORYLATED PEPTIDES DETERMINED BY A COMPETITION ASSAY OR SURFACE-PLASMON RESONANCE
Autore:
PAYNE G; SHOELSON SE; GISH GD; PAWSON T; WALSH CT;
Indirizzi:
HARVARD UNIV,SCH MED,DEPT BIOL CHEM & MOLEC PHARMACOL BOSTON MA 02115 BRIGHAM & WOMENS HOSP,DEPT MED,JOSLIN DIABET CTR,DIV RES BOSTON MA 02115 MT SINAI HOSP,SAMUEL LUNENFELD RES INST TORONTO M5G 1X5 ONTARIO CANADA
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 11, volume: 90, anno: 1993,
pagine: 4902 - 4906
SICI:
0027-8424(1993)90:11<4902:KOPAPS>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOPLASMIC PROTEIN; SIGNAL TRANSDUCTION; KINASE; PP60C-SRC; ACTIVATION; SH2; TRANSFORMATION; PURIFICATION; ELEMENTS; TERMINUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
G. Payne et al., "KINETICS OF P56(LCK) AND P60(SRC) SRC HOMOLOGY-2 DOMAIN BINDING TO TYROSINE-PHOSPHORYLATED PEPTIDES DETERMINED BY A COMPETITION ASSAY OR SURFACE-PLASMON RESONANCE", Proceedings of the National Academy of Sciences of the United Statesof America, 90(11), 1993, pp. 4902-4906

Abstract

Src homology 2 (SH2) domains are phosphotyrosine-binding modules found within various signal-transducing proteins. We have determined hy I-125 competition assay and surface plasmon resonance that the SH2 domains of Src and Lck bind to a variety of phosphopeptides with similar affinity and specificity. Both bound with highest affinity [K(d(app)) almost-equal-to 3.7 nM; k(a) = 2.4 x 10(5) M-1.s-1; k(d) = 1.2 x 10(-3) s-1] a phosphopeptide having a Tyr(P)-Glu-Glu-Ile motif found in the hamster polyomavirus middle-sized tumor antigen. Intermediate affinity (5- to 40-fold lower) was observed with phosphopeptides corresponding to the regulatory domains of Src and Lck, containing Tyr527 and Tyr505, respectively. Lowest affinity (80- to 300-fold lower) was observed with phosphopeptides corresponding to phosphorylated tyrosines of GTPase-activating protein, insulin receptor substrate 1, and SH2 domain-containing protein-tyrosine-phosphatase 1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 07:16:29