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Titolo:
ENZYMATIC PRODUCTION OF D-BETA-ACETYLTHIOISOBUTYRIC ACID FROM METHYL DL-BETA-ACETYLTHIOISOBUTYRATE .4. PROCESS CONDITIONS FOR PRODUCTION OFD-BETA-ACETYLTHIOISOBUTYRIC ACID FROM METHYL DL-BETA-ACETYLTHIOISOBUTYRATE WITH THE CELLS OF PSEUDOMONAS-PUTIDA MR-2068
Autore:
SAKIMAE A; OZAKI E; TOYAMA H; OHSUGA N; NUMAZAWA R; MURAOKA I; HAMADA E; OHNISHI H;
Indirizzi:
MITSUBISHI RAYON CO LTD,CENT RES LABS,20-1 MIYUKI CHO OTAKE HIROSHIMA73906 JAPAN
Titolo Testata:
Bioscience, biotechnology, and biochemistry
fascicolo: 5, volume: 57, anno: 1993,
pagine: 782 - 786
SICI:
0916-8451(1993)57:5<782:EPODAF>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANGIOTENSIN-CONVERTING ENZYME; INHIBITORS; DESIGN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
9
Recensione:
Indirizzi per estratti:
Citazione:
A. Sakimae et al., "ENZYMATIC PRODUCTION OF D-BETA-ACETYLTHIOISOBUTYRIC ACID FROM METHYL DL-BETA-ACETYLTHIOISOBUTYRATE .4. PROCESS CONDITIONS FOR PRODUCTION OFD-BETA-ACETYLTHIOISOBUTYRIC ACID FROM METHYL DL-BETA-ACETYLTHIOISOBUTYRATE WITH THE CELLS OF PSEUDOMONAS-PUTIDA MR-2068", Bioscience, biotechnology, and biochemistry, 57(5), 1993, pp. 782-786

Abstract

The process conditions concerned with the production of D-beta-acetylthioisobutyric acid were investigated. Methyl DL-beta-acetylthioisobutyrate as an enzyme substrate was produced from methyl methacrylate in 96.6% yield by allowing 1.0 mol of methyl methacrylate to react with 1.6 mol of thioacetic acid at 80-degrees-C for 6h. Through the study ofhydrolytic reaction of methyl DL-beta-acetylthioisobutyrate with the cells of Pseudomonas putida MR-2068, it was found that D-beta-acetylthioisobutyric acid having more than 98% (e.e.) optical purity was obtained when the reaction was done below pH 7.5 and below 50-degrees-C. For example. 10% (w/v) methyl DL-beta-acetylthioisobutyrate in the reaction mixture was asymmetrically hydrolyzed by 0.2% (w/v) cells at 45-degrees-C at pH 7.0 for 18 h to give DAT having 98.2% (e.e.) optical purity in 49.7% yield. To prevent the decompositon and racemization of D-beta-acetylthioisobutyric acid in the purification process, the use ofa thin-film distillation apparatus for a continuous distillation was proposed to be an effective purification method.

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Documento generato il 07/04/20 alle ore 04:04:47