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Titolo:
A 5' PORTION OF THE ICAM-1 GENE CONFERS TISSUE-SPECIFIC DIFFERENTIAL EXPRESSION LEVELS AND CYTOKINE RESPONSIVENESS
Autore:
CORNELIUS LA; TAYLOR JT; DEGITZ K; LI LJ; LAWLEY TJ; CAUGHMAN SW;
Indirizzi:
EMORY UNIV,SCH MED,DEPT DERMATOL,5001 WOODRUFF MEM BLDG ATLANTA GA 30322 EMORY UNIV,SCH MED,DEPT DERMATOL,5001 WOODRUFF MEM BLDG ATLANTA GA 30322 EMORY UNIV,SCH MED,VET ADM MED CTR ATLANTA GA 30322 UNIV MUNICH,DEPT DERMATOL W-8000 MUNICH 2 GERMANY
Titolo Testata:
Journal of investigative dermatology
fascicolo: 6, volume: 100, anno: 1993,
pagine: 753 - 758
SICI:
0022-202X(1993)100:6<753:A5POTI>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-ENDOTHELIAL CELLS; ADHESION MOLECULE-1 ICAM-1; TUMOR-NECROSIS-FACTOR; INTERFERON-GAMMA; MONOCLONAL-ANTIBODIES; HUMAN KERATINOCYTES; B-CELLS; T-CELLS; KAPPA-B; INTERLEUKIN-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
L.A. Cornelius et al., "A 5' PORTION OF THE ICAM-1 GENE CONFERS TISSUE-SPECIFIC DIFFERENTIAL EXPRESSION LEVELS AND CYTOKINE RESPONSIVENESS", Journal of investigative dermatology, 100(6), 1993, pp. 753-758

Abstract

Intercellular adhesion molecule-1 (ICAM-1), a cell-adhesion molecule critically involved in leukocyte trafficking and adherence, displays tissue-specific and cytokine-specific expression profiles. Although human dermal microvascular endothelial cells (HDMEC) constitutively express ICAM-1, keratinocytes (HK) do not. Interleukin-1 (IL-1) upregulatesICAM-1 expression in HDMEC, but fails to do so in either HK or A431, a human squamous carcinoma cell line, even though both have IL-1 receptors and express ICAM-1 on exposure to other cytokines. We have previously characterized a human ICAM-1 genomic clone that contains the 5' flanking transcriptional regulatory region. To test the hypothesis thattissue- and cytokine-specific ICAM-1 gene expression results from theinteraction of constitutive and inducible tissue-specific trans-acting factors with distinct cis-elements of the ICAM-1 gene, various ICAM-1-based reporter gene (CAT) plasmids were constructed. Transcriptionalactivity of these various constructs was assessed after transient transfection into HDMEC and A431. A critical ICAM-1 region was identifiedthat conferred enhanced expression of CAT in HDMEC and suppressed expression of CAT in A431. This same region further enhanced CAT expression in transfected HDMEC treated with IL-1alpha, yet no such enhancement was seen with IL-1 treatment of identically transfected A431. However, treatment of A431 transfectants with IFNgamma did result in enhanced CAT expression, demonstrating reversal of A431 cell context suppression of the ICAM-1-based reporter gene construct. These data implicate the existence of both tissue- and cytokine-specific responsive elements in the 5' flanking region of the ICAM- 1 gene and demonstrate that regulatory effects directed by such elements are dependent upon their cellular context. Moreover, they provide the basis for identification of specific cis-acting genetic elements, the trans-acting factors with which they interact, and the molecular mechanisms by which they regulate transcription of the ICAM-1 gene.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/07/20 alle ore 07:56:24