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Titolo:
A MITOCHONDRIAL HEAT-SHOCK PROTEIN FROM CRITHIDIA-FASCICULATA
Autore:
EFFRON PN; TORRI AF; ENGMAN DM; DONELSON JE; ENGLUND PT;
Indirizzi:
JOHNS HOPKINS SCH MED,DEPT BIOL CHEM BALTIMORE MD 21205 JOHNS HOPKINS SCH MED,DEPT BIOL CHEM BALTIMORE MD 21205 HOWARD HUGHES MED INST IOWA CITY IA 00000 NORTHWESTERN UNIV,DEPT PATHOL CHICAGO IL 60611 NORTHWESTERN UNIV,DEPT MICROBIOL IMMUNOL CHICAGO IL 60611 UNIV IOWA,DEPT BIOCHEM IOWA CITY IA 52242 JOHNS HOPKINS SCH MED,DEPT INTERNAL MED BALTIMORE MD 21205
Titolo Testata:
Molecular and biochemical parasitology
fascicolo: 2, volume: 59, anno: 1993,
pagine: 191 - 200
SICI:
0166-6851(1993)59:2<191:AMHPFC>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
LAMBDA-DNA-REPLICATION; ESCHERICHIA-COLI DNAK; BACTERIOPHAGE-LAMBDA; SACCHAROMYCES-CEREVISIAE; NUCLEOPROTEIN STRUCTURES; MULTIGENE FAMILY; KINETOPLAST DNA; HSP70 FAMILY; MEMBER; BINDING;
Keywords:
CRITHIDIA-FASCICULATA; HEAT SHOCK PROTEIN; HSP70; KINETOPLAST;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
P.N. Effron et al., "A MITOCHONDRIAL HEAT-SHOCK PROTEIN FROM CRITHIDIA-FASCICULATA", Molecular and biochemical parasitology, 59(2), 1993, pp. 191-200

Abstract

MCP72 is a mitochondrial hsp70 protein from the trypanosomatid Crithidia fasciculata. An MCP72 cDNA clone was isolated from a C.fasciculatacDNA library by screening with antiserum specific for the homologous protein of Trypanosoma cruzi [9]. The MCP72 cDNA encodes a polypeptideof 663 amino acids which is 84% identical to the Trypanosoma cruzi protein and 56% identical to the Escherichia coli hsp70 protein DnaK. MCP72 is less similar to other hsp70 proteins. Native MCP72 was purifiedto homogeneity by ATP-agarose affinity chromatography. Comparison of its N-terminal amino acid sequence with that deduced from the cDNA sequence shows that 20 amino acid residues had been cleaved from the N-terminus; this sequence probably represents a mitochondrial import signal which is cleaved during translocation into the mitochondrion. Fluorescence microscopy, using antibodies specific for MCP72, indicates thatthe protein is concentrated in a region of the mitochondrial matrix which surrounds the kinetoplast.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 07:54:16