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Titolo:
AMINO-ACYLATION SITE MUTATIONS IN AMINO ACID-ACTIVATING DOMAINS OF SURFACTIN SYNTHETASE - EFFECTS ON SURFACTIN PRODUCTION AND COMPETENCE DEVELOPMENT IN BACILLUS-SUBTILIS
Autore:
DSOUZA C; NAKANO MM; CORBELL N; ZUBER P;
Indirizzi:
LOUISIANA STATE UNIV,MED CTR,DEPT BIOCHEM & MOLEC BIOL,1501 KINGS HIGHWAY SHREVEPORT LA 71130 LOUISIANA STATE UNIV,MED CTR,DEPT BIOCHEM & MOLEC BIOL,1501 KINGS HIGHWAY SHREVEPORT LA 71130
Titolo Testata:
Journal of bacteriology
fascicolo: 11, volume: 175, anno: 1993,
pagine: 3502 - 3510
SICI:
0021-9193(1993)175:11<3502:ASMIAA>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
GRAMICIDIN-S SYNTHETASE-2; ESCHERICHIA-COLI; NUCLEOTIDE-SEQUENCE; SIGNAL TRANSDUCTION; PEPTIDE ANTIBIOTICS; REGULATORY GENE; BIOSYNTHESIS; EXPRESSION; BREVIS; OPERON;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
C. Dsouza et al., "AMINO-ACYLATION SITE MUTATIONS IN AMINO ACID-ACTIVATING DOMAINS OF SURFACTIN SYNTHETASE - EFFECTS ON SURFACTIN PRODUCTION AND COMPETENCE DEVELOPMENT IN BACILLUS-SUBTILIS", Journal of bacteriology, 175(11), 1993, pp. 3502-3510

Abstract

The part of the srfA operon of Bacillus subtilis that contains the region required for competence development is composed of the first fouramino acid-activating domains which are responsible for the incorporation of Glu, Leu, D-Leu, and Val into the peptide moiety of the lipopeptide surfactin. Ser-to-Ala substitutions were made in the amino-acylation site of each domain, and their effects on surfactin production and competence development were examined. All of the mutations conferreda surfactin-negative phenotype, supporting the finding that the conserved Ser in the amino-acylation site is required for peptide synthesis. However, none of the mutations affected significantly competence development or the expression of a lacZ fusion to the late competence operon comG. This, coupled with recent findings that only the fourth, Val-activating, domain is required for competence, suggests that some activity, other than amino-acylation and perhaps unrelated to peptide synthesis, possessed by the fourth domain is involved in the role of srfAin regulating competence development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/11/20 alle ore 21:33:16