Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
EFFECTS OF PERFUSION, FIXATION, AND FREEZING ON THE CONCENTRATION ANDCHEMICAL FORM OF MERCURY IN THE BRAIN OF RATS EXPOSED TO METHYLMERCURY
Autore:
MOTTET K; FRIBERG L; VAHTER M; BODY R; LIND B; SHEN D; BURBACHER T;
Indirizzi:
UNIV WASHINGTON,DEPT PATHOL ENVIRONM HLTH & PHARMACEUT,SM-30 SEATTLE WA 98195 KAROLINSKA INST,INST ENVIRONM MED S-10401 STOCKHOLM 60 SWEDEN KAROLINSKA INST,DEPT ENVIRONM HYG S-10401 STOCKHOLM 60 SWEDEN
Titolo Testata:
The Journal of trace elements in experimental medicine
fascicolo: 1, volume: 6, anno: 1993,
pagine: 23 - 30
SICI:
0896-548X(1993)6:1<23:EOPFAF>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
STORAGE; BLOOD;
Keywords:
METHYLMERCURY; INORGANIC MERCURY; SPECIATION IN BRAIN; PERFUSION FIXATION; FREEZING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
K. Mottet et al., "EFFECTS OF PERFUSION, FIXATION, AND FREEZING ON THE CONCENTRATION ANDCHEMICAL FORM OF MERCURY IN THE BRAIN OF RATS EXPOSED TO METHYLMERCURY", The Journal of trace elements in experimental medicine, 6(1), 1993, pp. 23-30

Abstract

These experiments were done to determine whether perfusion fixation or freezing alters the ratio of methyl and inorganic mercury in the brain. Thirty adult female Sprague-Dawley rats were divided into four groups; nine in each of two perfusion experimental groups, six in a freezing group and six control animals. The experimentals received two doses (5 mg/Hg kg) of methylmercury (MeHg) intraperitoneally 48 hours apart. Ninety-six hours after the first injection, nine of the MeHg animals were transcardially perfused with normal saline and Millonig's fixative, nine with normal saline perfusion alone, and six were not perfused but the brains were frozen. Blood and brain were analyzed for total mercury and brain samples also for speciation. The percentage of inorganic Hg in the brain did not differ across the groups. The nonperfusedfrozen only brains had significantly higher mercury levels than the saline only or saline plus Millonig's fixative groups that can be largely accounted for by the removal of mercury in the blood within the brain blood vessels by perfusion. There was no significant change in the organic to inorganic ratio of brain mercury. The use of fixatives, fixation procedures, and storage of tissue are discussed. We conclude that under appropriate conditions tissue specimens can be fixed or frozenwithout change in ratio of methyl and inorganic mercury.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 20:11:04