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Titolo:
G1 EXPRESSION AND MULTISTAGE DYNAMICS OF CYCLIN-A IN HUMAN OSTEOSARCOMA CELLS
Autore:
CARBONAROHALL D; WILLIAMS R; WU LT; WARBURTON D; ZEICHNERDAVID M; MACDOUGALL M; TOLO V; HALL F;
Indirizzi:
CHILDRENS HOSP,DIV ORTHOPAED SURG,4650 SUNSET BLVD LOS ANGELES CA 90054 CHILDRENS HOSP,DIV ORTHOPAED SURG,4650 SUNSET BLVD LOS ANGELES CA 90054 UNIV SO CALIF,SCH MED LOS ANGELES CA 90033 CHILDRENS HOSP,DIV NEONATAL & PEDIAT PULM LOS ANGELES CA 90054 UNIV SO CALIF,SCH DENT LOS ANGELES CA 90089 UNIV SO CALIF,CTR CRANIOFACIAL MOLEC BIOL LOS ANGELES CA 90089 UNIV SO CALIF,SCH PHARM LOS ANGELES CA 90089 UNIV SO CALIF,DEPT MOLEC PHARMACOL & TOXICOL LOS ANGELES CA 90089
Titolo Testata:
Oncogene
fascicolo: 6, volume: 8, anno: 1993,
pagine: 1649 - 1659
SICI:
0950-9232(1993)8:6<1649:GEAMDO>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
RETINOBLASTOMA GENE-PRODUCT; P34CDC2 PROTEIN-KINASE; MATURATION-PROMOTING FACTOR; DNA-BINDING PROTEIN; CDC2 PROTEIN; TRANSCRIPTION FACTOR; HISTONE-H1 KINASE; RB PROTEIN; M-PHASE; PHOSPHORYLATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
77
Recensione:
Indirizzi per estratti:
Citazione:
D. Carbonarohall et al., "G1 EXPRESSION AND MULTISTAGE DYNAMICS OF CYCLIN-A IN HUMAN OSTEOSARCOMA CELLS", Oncogene, 8(6), 1993, pp. 1649-1659

Abstract

Cyclin A was initially characterized as a 'mitotic cyclin', believed to function exclusively at the G2-to-M phase transition; however, recent studies have provided compelling evidence that cyclin A additionally functions earlier in the mammalian somatic cell cycle as a putative 'S-phase-promoting factor'. Moreover, numerous inconsistencies have arisen concerning the temporal induction, subcellular localization, subunit configuration, covalent modification and proteolytic destruction of cyclin A, as well as the physiological function of the cyclin A-associated protein kinase complexes. Utilizing precisely synchronized human MG-63 osteosarcoma cells, the present study demonstrates that cyclinA mRNA and protein are clearly expressed in late G1 prior to S-phase entry, as is cyclin A-associated kinase activity and concomitant phosphorylation of the Rb protein. A series of monospecific cyclin A antibodies were generated and utilized to confirm that multiple covalent modifications of cyclin A occur during the course of the cell cycle, and to characterize the subcellular dynamics in additional detail. Pharmacological blockade with mimosine was utilized to further delineate cyclin A expression and to distinguish the temporal induction from the mechanisms of enzyme activation. Subcellular fractionation and immunocytochemical staining localized nascent cyclin A to the cytoplasm, and revealed a distinct translocation to the nucleus during the G1-to-S phasetransition. The results of these studies support a multistage model of cyclin A metabolism and enzyme activation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 02:02:16