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Titolo:
INDUCTION OF ACROSOME REACTIONS OF CANINE SPERM BY HOMOLOGOUS ZONA-PELLUCIDA
Autore:
KAWAKAMI E; VANDEVOORT CA; MAHIBROWN CA; OVERSTREET JW;
Indirizzi:
UNIV CALIF DAVIS,SCH MED,CALIF REG PRIMATE RES CTR,SUBER HOUSE DAVIS CA 95616 UNIV CALIF DAVIS,SCH MED,CALIF REG PRIMATE RES CTR,SUBER HOUSE DAVIS CA 95616 UNIV CALIF DAVIS,SCH MED,DEPT OBSTET & GYNECOL,DIV REPROD BIOL & MED DAVIS CA 95616
Titolo Testata:
Biology of reproduction
fascicolo: 4, volume: 48, anno: 1993,
pagine: 841 - 845
SICI:
0006-3363(1993)48:4<841:IOAROC>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE GAMETE INTERACTIONS; REACTED HUMAN-SPERM; FERTILIZATION INVITRO; ACTIVE IMMUNIZATION; SPERMATOZOA; PENETRATION; BINDING; CAPACITATION; INTACT; GLYCOPROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
E. Kawakami et al., "INDUCTION OF ACROSOME REACTIONS OF CANINE SPERM BY HOMOLOGOUS ZONA-PELLUCIDA", Biology of reproduction, 48(4), 1993, pp. 841-845

Abstract

In this study the induction of the acrosome reaction of canine sperm by homologous zona pellucida (ZP) was examined. Twelve semen samples obtained from 6 normal beagle dogs were evaluated after sperm incubation in vitro with canine capacitation medium (CCM). Washed sperm were preincubated at 37-degrees-C in 5% CO2 in air for 4 and 7 h prior to experimental treatment. Sperm were co-incubated for 1 min with intact oocytes collected from canine ovaries. Half of the oocytes were then fixed, and the bound sperm were assessed for acrosome reactions through use of a polyclonal antisperm antiserum and indirect immunofluorescence. The remaining oocytes were incubated in sperm-free medium for an additional 1-h period, and the acrosomal status of sperm bound to the ZP was evaluated similarly. The percentage of acrosome-reacted sperm on the ZP increased significantly during the 1-h incubation period. In other experiments, capacitated canine sperm were incubated with heat-solubilized ZP for 1 h and their acrosomal status was determined using fluoresceinated Pisum sativum lectin. The percentages of acrosome-reacted sperm increased significantly in ZP solution compared with controls. These data demonstrate that intact and solubilized canine ZP are capable of inducing acrosome reactions of canine sperm.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 19:50:51