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Titolo:
VITRIFICATION OF BOVINE EMBRYOS IN A MIXTURE OF ETHYLENE-GLYCOL AND DIMETHYL-SULFOXIDE
Autore:
ISHIMORI H; SAEKI K; INAI M; NAGAO Y; ITASAKA J; MIKI Y; SEIKE N; KAINUMA H;
Indirizzi:
SNOW BRAND MILK PROD CO LTD,EMBRYO TRANSPLANTAT LAB,119 UENAE TOMAKOMAI HOKKAIDO 05913 JAPAN
Titolo Testata:
Theriogenology
fascicolo: 2, volume: 40, anno: 1993,
pagine: 427 - 433
SICI:
0093-691X(1993)40:2<427:VOBEIA>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTORS AFFECTING SURVIVAL; MOUSE EMBRYOS; CATTLE EMBRYOS; CRYOPRESERVATION; VIABILITY; CRYOPROTECTANT; EQUILIBRATION; PREGNANCIES; SHEEP;
Keywords:
BOVINE EMBRYOS; EMBRYO TRANSFER; VITRIFICATION; ETHYLENE GLYCOL; DIMETHYL SULFOXIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
H. Ishimori et al., "VITRIFICATION OF BOVINE EMBRYOS IN A MIXTURE OF ETHYLENE-GLYCOL AND DIMETHYL-SULFOXIDE", Theriogenology, 40(2), 1993, pp. 427-433

Abstract

The influence of equilibration time before vitrification on the viability of vitrified morula- to blastocyst-stage bovine embryos and in vivo viability of vitrified embryos following transfer to recipients were investigated. In experiment 1, the embryos were exposed to an equilibration solution (50% VSED) containing 12.5% v/v ethylene glycol and 12.5% v/v dimethyl sulfoxide in modified Dulbecco's phosphate buffered saline with 4 mg/ml BSA (m-PBS) for 1, 2 and 5 minutes at room temperature (22 to 24-degrees-C). The embryos were then placed in 15 mul vitrification solution (VSED) consisting of 25% v/v ethylene glycol and 25% v/v dimethyl sulfoxide in m-PBS and were loaded into 0.25 ml plasticstraws at room temperature. After 30 seconds, the straws were placed in liquid nitrogen (LN2) vapor for 2 minutes, plunged and stored in LN2. To thaw, the straws were warmed in water at 20-degrees-C for 15 seconds and the contents of the straws were expelled into a plastic dish. The embryos were diluted in 0.5 M sucrose + m-PBS for 5 minutes and were cultured in TCM-199 supplemented with bovine oviductal epithelial tissue. Viability of the embryos was assessed by the forming or reforming of the blastocoele after 24 hours of culture. High in vitro survival rates (73 approximately 90%) of vitrified embryos were obtained after 1 and 2 minute equilibrations, but was reduced (P < 0.05) after 5 minute equilibration. In Experiment 2, morula- to blastocyst-stage embryos were vitrified after 1 minute equilibration in 50% VSED and 30 seconds of exposure to VSED. The vitrified-warmed embryos were transferred to recipient heifers at 7 days after estrus (1 embryo per recipient). Five (38%) of 13 recipients that had received morulae and early blastocysts and 4 (40%) of 10 recipients that had received blastocysts were diagnosed as pregnant using ultrasonography 60 days following transfer.

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Documento generato il 18/09/20 alle ore 16:42:51