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Titolo:
MICROSATELLITE DELETIONS IN THE C-MYB TRANSCRIPTIONAL ATTENUATOR REGION ASSOCIATED WITH OVER-EXPRESSION IN COLON-TUMOR CELL-LINES
Autore:
THOMPSON MA; FLEGG R; WESTIN EH; RAMSAY RG;
Indirizzi:
PETER MACCALLUM CANC INST,LOCKED BAG 1,ST ANDREWS PL MELBOURNE VIC 3002 AUSTRALIA PETER MACCALLUM CANC INST MELBOURNE VIC 3002 AUSTRALIA ROYAL MELBOURNE HOSP,LUDWIG INST CANC RES PARKVILLE VIC 3050 AUSTRALIA VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT MED RICHMOND VA 23298
Titolo Testata:
Oncogene
fascicolo: 14, volume: 14, anno: 1997,
pagine: 1715 - 1723
SICI:
0950-9232(1997)14:14<1715:MDITCT>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
NONPOLYPOSIS COLORECTAL-CANCER; MESSENGER-RNA; MULTIPLE MECHANISMS; REPLICATION ERRORS; SUPPRESSOR PROTEIN; ADENOMATOUS POLYPS; SODIUM-BUTYRATE; GENE; DIFFERENTIATION; ELONGATION;
Keywords:
C-MYB; TRANSCRIPTIONAL ATTENUATION; COLON TUMOR CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
63
Recensione:
Indirizzi per estratti:
Citazione:
M.A. Thompson et al., "MICROSATELLITE DELETIONS IN THE C-MYB TRANSCRIPTIONAL ATTENUATOR REGION ASSOCIATED WITH OVER-EXPRESSION IN COLON-TUMOR CELL-LINES", Oncogene, 14(14), 1997, pp. 1715-1723

Abstract

In the hemopoietic system c-myb expression is required for proliferation of immature cells and its downregulation is required for differentiation. In colonic mucosa c-myb expression occurs at levels comparableto immature hemopoietic cells. Inhibition of c-myb expression in colon cell lines, using anti-sense oligonucleotides, indicates that c-myb expression is required for proliferation. However, the mechanism of c-myb regulation during colon cell differentiation has not been explored. Using the LIM1215 and CaCo-2 colon carcinoma cell lines induced to differentiate with sodium butyrate, we demonstrate that c-myb mRNA is down-regulated as an early event in differentiation by a mechanism involving transcriptional attenuation in intron 1. By analogy with procaryotic and eucaryotic genes, transcriptional attenuation probably occursin a region containing nineteen consecutive thymidine residues. Computer prediction of the secondary structure of the nascent mRNA chain encoded by this region suggests a strong potential for stem-loop formation. Sequence analysis of several colon tumour cell lines reveals mutations in this region that may disrupt transcriptional attenuation and result in the increased c-myb expression observed in colon tumours and tumour cell lines.

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Documento generato il 04/12/20 alle ore 19:48:14