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Titolo:
TRANSIENT INHIBITION OF FOOT-AND-MOUTH-DISEASE VIRUS-INFECTION OF BHK-21-CELLS BY ANTISENSE OLIGONUCLEOTIDES DIRECTED AGAINST THE 2ND FUNCTIONAL INITIATOR AUG
Autore:
GUTIERREZ A; RODRIGUEZ A; PINTADO B; SOBRINO F;
Indirizzi:
INIA,CIT,DEPT PROD ANIM,CTRA LA CORUNA KM 5 900 E-28040 MADRID SPAIN INIA E-28130 VALDEOLMOS SPAIN
Titolo Testata:
Antiviral research
fascicolo: 1, volume: 22, anno: 1993,
pagine: 1 - 13
SICI:
0166-3542(1993)22:1<1:TIOFVO>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN IMMUNODEFICIENCY VIRUS; PROTEIN-SYNTHESIS; PHOSPHOROTHIOATE ANALOGS; NUCLEOTIDE-SEQUENCE; ANTIVIRAL AGENTS; NONCODING REGION; RNA TRANSLATION; SPLICE JUNCTION; MESSENGER RNA-4; OLIGODEOXYNUCLEOTIDES;
Keywords:
FMDV; ANTISENSE OLIGONUCLEOTIDES; MICROINJECTION; TRANSLATION INHIBITION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
A. Gutierrez et al., "TRANSIENT INHIBITION OF FOOT-AND-MOUTH-DISEASE VIRUS-INFECTION OF BHK-21-CELLS BY ANTISENSE OLIGONUCLEOTIDES DIRECTED AGAINST THE 2ND FUNCTIONAL INITIATOR AUG", Antiviral research, 22(1), 1993, pp. 1-13

Abstract

The antiviral activity of antisense oligonucleotides corresponding todifferent regions of foot-and-mouth disease virus (FMDV) genome has been assessed in BHK-21 cells. The locations of the oligonucleotides used were: (i) two regions within the internal ribosome entry site (IRES), involved in the regulation of the translation initiation of the viral polyprotein; (ii) each of the two functional initiator AUGs; (iii) an internal sequence of P2A gene; and (iv) a region at the 3' end non-coding region. Cytoplasmic microinjection of oligodeoxyribonucleotidesand oligoribonucleotides complementary to the second AUG resulted in a transient inhibition of viral VP1 expression in infected cells. Significant inhibitions, ranging from 35 to 52%, were obtained at 5 h post-infection using oligonucleotide concentrations of 125 muM and higher. The extent and duration of this inhibition seemed to be mediated by both a rapid transport to the nucleus and the short half-life of the oligonucleotide. This inhibition of FMDV protein synthesis was correlated with a reduction of virus yield of about 50%, as observed after the addition to the cell culture of an oligodeoxyribonucleotide phosphorothioate complementary to the second AUG.

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Documento generato il 25/09/20 alle ore 09:38:12