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Titolo:
HUMORAL IMMUNE-RESPONSES IN VOLUNTEERS IMMUNIZED WITH IRRADIATED PLASMODIUM-FALCIPARUM SPOROZOITES
Autore:
EGAN JE; HOFFMAN SL; HAYNES JD; SADOFF JC; SCHNEIDER I; GRAU GE; HOLLINGDALE MR; BALLOU WR; GORDON DM;
Indirizzi:
WALTER REED ARMY INST RES,DEPT IMMUNOL WASHINGTON DC 20307 WALTER REED ARMY INST RES,DEPT IMMUNOL WASHINGTON DC 20307 WALTER REED ARMY INST RES,DEPT ENTOMOL WASHINGTON DC 20307 USN,MED RES INST,MALARIA PROGRAM BETHESDA MD 20814 UNIV GENEVA,CMU,DEPT PATHOL CH-1211 GENEVA 4 SWITZERLAND BIOMED RES INST ROCKVILLE MD 00000
Titolo Testata:
The American journal of tropical medicine and hygiene
fascicolo: 2, volume: 49, anno: 1993,
pagine: 166 - 173
SICI:
0002-9637(1993)49:2<166:HIIVIW>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
MALARIA VACCINE TRIALS; CD8+ T-CELLS; CIRCUMSPOROZOITE PROTEIN; SURFACE-ANTIGEN; ANTIBODIES; MICE; EFFICACY; GENE; PROTECTION; INHIBITION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
J.E. Egan et al., "HUMORAL IMMUNE-RESPONSES IN VOLUNTEERS IMMUNIZED WITH IRRADIATED PLASMODIUM-FALCIPARUM SPOROZOITES", The American journal of tropical medicine and hygiene, 49(2), 1993, pp. 166-173

Abstract

Volunteers immunized with gamma-irradiated Plasmodium falciparum sporozoites serve as the gold standard for protective immunity against mosquito-borne malaria transmission and provide a relevant model for studying protective immune effector mechanisms. During a 7-12-month period, we immunized four volunteers via the bites of irradiated, infected mosquitoes. Following these exposures to attentuated sporozoites, all four volunteers developed antibodies to sporozoites as measured by an immunofluorescence assay and by an enzyme-linked immunosorbent assay using the circumsporozoite (CS) protein repeat-based molecule R32LR as capture antigen. Three volunteers also developed antibodies against thenonrepeating (flanking) regions of the CS protein; the level of theseantibodies paralleled the serum activity to inhibit sporozoite invasion of hepatoma cells in vitro. These three volunteers were protected against malaria transmitted by the bites of five infected mosquitoes. Two of these protected volunteers received additional immunizing doses of irradiated sporozoites and were subsequently protected against challenge with a heterologous P. falciparum clone. No detectable fluctuations were observed in circulating levels of tumor necrosis factor, interferon-gamma, or interleukin-6 during the course of this study. Analysis of the humoral and cellular immune responses of these protected volunteers is expected to yield important clues to additional targets of immunity against the pre-erythrocytic stages of malaria parasites.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 01:51:26