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Titolo:
QUANTITATION OF MEMBRANE-RECEPTOR DISTRIBUTIONS BY IMAGE CORRELATION SPECTROSCOPY - CONCEPT AND APPLICATION
Autore:
PETERSEN NO; HODDELIUS PL; WISEMAN PW; SEGER O; MAGNUSSON KE;
Indirizzi:
UNIV WESTERN ONTARIO,DEPT CHEM LONDON N6A 5B7 ONTARIO CANADA LINKOPING UNIV,FAC HLTH SCI,DEPT CELL BIOL S-58183 LINKOPING SWEDEN LINKOPING UNIV,FAC HLTH SCI,DEPT ELECT ENGN S-58183 LINKOPING SWEDEN LINKOPING UNIV,FAC HLTH SCI,DEPT MED MICROBIOL S-58183 LINKOPING SWEDEN
Titolo Testata:
Biophysical journal
fascicolo: 3, volume: 65, anno: 1993,
pagine: 1135 - 1146
SICI:
0006-3495(1993)65:3<1135:QOMDBI>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMAL GROWTH-FACTOR; FLUORESCENCE CORRELATION SPECTROSCOPY; LATERAL DIFFUSION; A431 CELLS; DIRECT VISUALIZATION; HUMAN-FIBROBLASTS; AGGREGATION; BINDING; INTERNALIZATION; ANTIBODIES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
N.O. Petersen et al., "QUANTITATION OF MEMBRANE-RECEPTOR DISTRIBUTIONS BY IMAGE CORRELATION SPECTROSCOPY - CONCEPT AND APPLICATION", Biophysical journal, 65(3), 1993, pp. 1135-1146

Abstract

Measurement of receptor distributions on cell surfaces is one important aspect of understanding the mechanism whereby receptors function. In recent years, scanning fluorescence correlation spectroscopy has emerged as an excellent tool for making quantitative measurements of cluster sizes and densities. However, the measurements are slow and usually require fixed preparations. Moreover, while the precision is good, the accuracy is limited by the relatively small amount of information in each measurement, such that many are required. Here we present a novel extension of the scanning correlation spectroscopy that solves a number of the present problems. The new technique, which we call image correlation spectroscopy, is based on quantitative analysis of confocalscanning laser microscopy images. Since these can be generated in a matter of a second or so, the measurements become more rapid. The imageis collected over a large cell area so that more sampling is done, improving the accuracy. The sacrifice is a lower resolution in the sampling, which leads to a lower precision. This compromise of precision infavor of speed and accuracy still provides an enormous advantage for image correlation spectroscopy over scanning correlation spectroscopy. The present work demonstrates the underlying theory, showing how the principles can be applied to measurements on standard fluorescent beads and changes in distribution of receptors for platelet-derived growthfactor on human foreskin fibroblasts.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 05:35:25