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Titolo:
PROTEOLYTIC ACTIVITY IN THE SEMINAL PLASMA OF THE MUD CRAB, SCYLLA-SERRATA (FORSKAL)
Autore:
JAYASANKAR V; SUBRAMONIAM T;
Indirizzi:
UNIV MADRAS,DEPT ZOOL,UNIT INVERTEBRATE REPROD,GUINDY CAMPUS MADRAS 600025 TAMIL NADU INDIA UNIV MADRAS,DEPT ZOOL,UNIT INVERTEBRATE REPROD MADRAS 600025 TAMIL NADU INDIA
Titolo Testata:
Comparative biochemistry and physiology. B. Comparative biochemistry
fascicolo: 3, volume: 116, anno: 1997,
pagine: 347 - 352
SICI:
0305-0491(1997)116:3<347:PAITSP>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
SODIUM DODECYL-SULFATE; POLYACRYLAMIDE GELS; SECRETIONS; FLUID; PROTEINASE;
Keywords:
BRACHYURA; CASEIN; CRUSTACEAN; HPLC; PAGE; PROTEASE; SCYLLA SERRATA; SEMINAL PLASMA; SPERM PLUG;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
V. Jayasankar e T. Subramoniam, "PROTEOLYTIC ACTIVITY IN THE SEMINAL PLASMA OF THE MUD CRAB, SCYLLA-SERRATA (FORSKAL)", Comparative biochemistry and physiology. B. Comparative biochemistry, 116(3), 1997, pp. 347-352

Abstract

The protein composition and proteolytic activity of the seminal plasma of the brachyuran crab, Scylla serrata, were studied. The seminal plasma proteins are mainly glycoproteinaceous, many of them lying in thelower molecular weight range as evidenced by high performance liquid chromatography (HPLC). Protease activity was detected using casein as the substrate. The enzyme was highly active in the pH range 7.0-9.0, exhibiting maximum activity at pH 8.0. The enzyme was active at temperatures up to 40 degrees C. Enzyme activity was inhibited by the serine protease inhibitor Phenyl Methyl Sulfonyl Fluoride (PMSF) but not by the metalloprotease inhibitor Ethylene Diamine Tetra Acetic acid (EDTA)indicating that it is a serine protease. The effect of substrate concentration on the enzyme activity was also studied. The proteins were fractionated by gel filtration chromatography on Sephadex G-200 column. A clear zone of proteolysis was detected against a stained backgroundin the third fraction when electrophoresed on casein-containing polyacrylamide gel. The molecular weight of this fraction was determined tobe approximately 6000D. The protease activity in the seminal plasma may be related to the formation and/or dissolution of sperm plug formedduring mating in these brachyuran crabs. (C) 1997 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 21:46:44